脳エピトランスクリプトミクス研究チーム

研究内容

RNAの時空間的な制御機構「エピトランスクリプトミクス」の中枢神経系における役割解明を目指します。特に学習・記憶、認知機能に焦点を当て、オミックス解析、蛍光イメージング、細胞生物学的手法、マウスモデル系によりアプローチします。

研究テーマ

• シナプス機能におけるRNA修飾の役割の解明
• 分子から行動までの各層におけるRNA修飾読み取り分子の役割の解明
• 各ライフステージにおけるRNA修飾状況の探索

主要論文

• Madugalle SU, Meyer K, Wang DO, Bredy TW.
  RNA N⁶-Methyladenosine and the Regulation of RNA Localization and Function in the Brain.
  Trends in Neurosciences 43, 1011-1023 (2020) doi: 10.1016/j.tins.2020.09.005
• Wang DO.
  RNA modifications in the central nervous system.
  Oxford Handbook on Neuronal Protein synthesis doi: 10.1093/oxfordhb/9780190686307.013.23
• Roy R, Shiina N, Wang DO.
  More dynamic, more quantitative, unexpectedly intricate: advanced understanding on synaptic RNA localization in learning and memory.
  Neurobiology of Learning and Memory 168, 107149 (2020)
• Wang DO.
  Mapping m⁶A and m¹A with mutational signatures.
  Nature Methods (2019) doi: 10.1038/s41592-019-0636-z
• Kashida S, Wang DO, Saito H, Gueroui Z.
  Nanoparticle-based local translation reveals mRNA as translation-coupled scaffold with anchoring function.
  Proceedings of the National Academy of Sciences of the United States of America 116(27), 13346-13351 (2019)
• Merkurjev D, Hong WT, Iida K, et al.
  Synaptic N⁶ methyladenosine (m⁶A) reveals functional partitioning of localized transcripts.
  Nature Neuroscience 21, 1004–1014 (2018)
• Oomoto I, Hirano-Suzuki A, Umeshima H, et al.
  ECHO-liveFISH: in vivo RNA Labeling Reveals Dynamic Regulation of Nuclear RNA Foci in Living Tissues.
  Nucleic Acids Research (2015) doi: 10.1093/nar/gkv614
• Meer E, Wang DO, Kim SM, et al.
  Identification of a cis-element that localizes mRNA to synapses.
  Proceedings of the National Academy of Sciences of the United States of America 109(12), 4639-44 (2012)
• Wang DO, Martin KC, Zukin RS.
  Spatially restricting gene expression by local translation at synapses.
  Trends in Neurosciences 33(4), 173-82 (2010)
• Wang DO, Kim SM, Zhao Y, et al.
  Synapse- and stimulus-specific local translation during long-term neuronal plasticity.
  Science 324(5934), 1536-40 (2009)