脳エピトランスクリプトミクス研究チーム
研究内容
RNAの時空間的な制御機構「エピトランスクリプトミクス」の中枢神経系における役割解明を目指します。特に学習・記憶、認知機能に焦点を当て、オミックス解析、蛍光イメージング、細胞生物学的手法、マウスモデル系によりアプローチします。
研究テーマ
- シナプス機能におけるRNA修飾の役割の解明
- 分子から行動までの各層におけるRNA修飾読み取り分子の役割の解明
- 各ライフステージにおけるRNA修飾状況の探索
主要論文
- Madugalle SU, Meyer K, Wang DO, Bredy TW.
RNA N6-Methyladenosine and the Regulation of RNA Localization and Function in the Brain.
Trends in Neurosciences 43, 1011-1023 (2020) doi: 10.1016/j.tins.2020.09.005 - Wang DO.
RNA modifications in the central nervous system.
Oxford Handbook on Neuronal Protein synthesis doi: 10.1093/oxfordhb/9780190686307.013.23 - Roy R, Shiina N, Wang DO.
More dynamic, more quantitative, unexpectedly intricate: advanced understanding on synaptic RNA localization in learning and memory.
Neurobiology of Learning and Memory 168, 107149 (2020) - Wang DO.
Mapping m6A and m1A with mutational signatures.
Nature Methods (2019) doi: 10.1038/s41592-019-0636-z - Kashida S, Wang DO, Saito H, Gueroui Z.
Nanoparticle-based local translation reveals mRNA as translation-coupled scaffold with anchoring function.
Proceedings of the National Academy of Sciences of the United States of America 116(27), 13346-13351 (2019) - Merkurjev D, Hong WT, Iida K, et al.
Synaptic N6 methyladenosine (m6A) reveals functional partitioning of localized transcripts.
Nature Neuroscience 21, 1004–1014 (2018) - Oomoto I, Hirano-Suzuki A, Umeshima H, et al.
ECHO-liveFISH: in vivo RNA Labeling Reveals Dynamic Regulation of Nuclear RNA Foci in Living Tissues.
Nucleic Acids Research (2015) doi: 10.1093/nar/gkv614 - Meer E, Wang DO, Kim SM, et al.
Identification of a cis-element that localizes mRNA to synapses.
Proceedings of the National Academy of Sciences of the United States of America 109(12), 4639-44 (2012) - Wang DO, Martin KC, Zukin RS.
Spatially restricting gene expression by local translation at synapses.
Trends in Neurosciences 33(4), 173-82 (2010) - Wang DO, Kim SM, Zhao Y, et al.
Synapse- and stimulus-specific local translation during long-term neuronal plasticity.
Science 324(5934), 1536-40 (2009)