チームリーダー
難波 啓一*
D.Eng.
超分子システム動態研究チーム
[2022年3月 終了]
*現在の連絡先 :keiichi[at]fbs.osaka-u.ac.jp
[at]を@に変えてください
細菌べん毛や筋フィラメントなど、生体や細胞の運動に関わる生体超分子モーターの機能・構造・動作機構を解明するため、クライオ電子顕微鏡やＸ線回折法に光学ナノ計測法、遺伝子工学、生化学的解析法などを組合せ、生体超分子の立体構造やダイナミックな動きを高時間空間分解能で解析します。そのために必要なクライオ電子顕微鏡法や光学ナノ計測法の開発も独自に進めます。
研究テーマ
- 細菌べん毛や筋フィラメントなど生体超分子モーターの機能・構造・動作機構
- クライオ電子顕微鏡による生体超分子立体構造の高速高分解能解析法の開発
主要論文
Fujii T, Matsunami H, Inoue Y, Namba K.
Evidence for the hook supercoiling mechanism of the bacterial flagellum.
Biophysics and Physicobiology
15, 28-32 (2018)
doi: 10.2142/biophysico.15.0_28
Sakai T, Inoue Y, Terahara N, et al.
A triangular loop of domain D1 of FlgE is essential for hook assembly but not for the mechanical function.
Biochemical and Biophysical Research Communications
495, 1789-1794 (2018)
doi: 10.1016/j.bbrc.2017.12.037
Kinoshita M, Namba K, Minamino T.
Effect of a clockwise-locked deletion in FliG on the FliG ring structure of the bacterial flagellar motor.
Genes to cells : devoted to molecular & cellular mechanisms
23(3), 241-247 (2018)
doi: 10.1111/gtc.12565
Inoue Y, Morimoto YV, Namba K, Minamino T.
Novel insights into the mechanism of well-ordered assembly of bacterial flagellar proteins in Salmonella.
Scientific reports
8(1), 1787 (2018)
doi: 10.1038/s41598-018-20209-3
Terahara N, Kodera N, Uchihashi T, et al.
Na+-induced structural transition of MotPS for stator assembly of Bacillus flagellar motor.
Science Advances
3, eaao0419 (2017)
doi: 10.1126/sciadv.aao4119
Pourjaberi SNS, Terahara N, Namba K, et al.
The role of a cytoplasmic loop of MotA in load-dependent assembly and disassembly dynamics of the MotA/B stator complex in the bacterial flagellar motor.
Molecular Microbiology
106, 646-658 (2017)
doi: 10.1111/mmi.13843
Fukumura T, Makino F, Dietsche T, et al.
Assembly and stoichiometry of the core structure of the bacterial flagellar type III export gate complex.
PLOS Biology
15(8), e2002281(22pp) (2017)
doi: 10.1371/journal.pbio.2002281
Kinoshita M, Aizawa S, Inoue Y, et al.
The role of intrinsically disordered C-terminal region of FliK in substrate specificity switching of the bacterial flagellar type III export apparatus.
Molecular Microbiology
105, 572-588 (2017)
doi: 10.1111/mmi.13718
Hiraoka KD, Morimoto YV, Inoue Y, et al.
Straight and rigid flagellar hook made by insertion of the FlgG specific sequence into FlgE.
Scientific Reports
7, 46723(8pp) (2017)
doi: 10.1038/srep46723
Terahara N, Noguchi Y, Nakamura S, et al.
Load- and polysaccharide-dependent activation of the Na+-type MotPS stator in the Bacillus subtilis flagellar motor.
Scientific Reports
7, 46081(11pp) (2017)
doi: 10.1038/srep46081
Renault TT, Abraham AO, Bergmiller T, et al.
Bacterial flagella grow through an injection-diffusion mechanism.
eLife
6, e23136 (2017)
doi: 10.7554/eLife.23136
