Laboratory for Supramolecular System Dynamics Research | RIKEN BDR

Laboratory for Supramolecular System Dynamics Research

Team Leader

Keiichi NambaD.Eng.

  • Location:Osaka
  • E-mail:keiichi[at]fbs.osaka-u.ac.jpPlease replace [at] with @.
  • Lab Website

Understanding the structures and mechanisms of biomolecular motors by electron cryomicroscopy

Research Summary

To gain deep understanding of the mechanisms of biomolecular motor protein assemblies, such as bacterial flagella and actomyosins, we make combined use of electron cryomicroscopy and image analysis with X-ray diffraction and optical nanophotometry as well as genetic engineering and protein biochemistry to visualize their three-dimensional structures and dynamics at high-temporal and spatial resolution. We also develop advanced technologies of electron cryomicroscopy and image analysis as well as optical nanophotometry on our own.

Research Theme

  • Structures and functions of biomolecular motor assemblies such as bacterial flagella and actomyosins
  • Technical development in electron cryomicroscopy and image analysis for high-resolution, high-throughput structural analysis

Main Publications List

  • Kinoshita, M., Namba, K., Minamino, T.
    Effect of a clockwise-locked deletion in FliG on the FliG ring structure of the bacterial flagellar motor.
    Genes to Cells in press doi:10.1111/gtc.12565
  • Fujii T, Matsunami H, Inoue Y, Namba K.
    Evidence for the hook supercoiling mechanism of the bacterial flagellum.
    Biophysics and Physicobiology 15. 28-32 (2018) doi: 10.2142/biophysico.15.0_28
  • Inoue Y, Morimoto YV, Namba K, Minamino T.
    Novel insights into the mechanism of well-ordered assembly of bacterial flagellar proteins in Salmonella.
    Scientific Reports
    8(1787). 11 (2018) doi: 10.1038/s41598-018-20209-3
  • Kinoshita, M., Furukawa, Y., Uchiyama, S., et al.
    Insight into adaptive remodeling of the rotor ring complex of the bacterial flagellar motor.
    Biochemical and Biophysical Research Communications 496. 12-17 (2018) doi:10.1016/j.bbrc.2017.12.118
  • Sakai T, Inoue Y, Terahara N, et al.
    A triangular loop of domain D1 of FlgE is essential for hook assembly but not for the mechanical function.
    Biochemical and Biophysical Research Communications 495. 1789-1794 (2018) doi:10.1016/j.bbrc.2017.12.037
  • Terahara N, Kodera N, Uchihashi T, et al.
    Na+-induced structural transition of MotPS for stator assembly of Bacillus flagellar motor.
    Science Advances 3. eaao0419 (2017) doi: 10.1126/sciadv.aao4119
  • Pourjaberi SNS, Terahara N, Namba K, et al.
    The role of a cytoplasmic loop of MotA in load-dependent assembly and disassembly dynamics of the MotA/B stator complex in the bacterial flagellar motor.
    Molecular Microbiology 106. 646-658 (2017) doi: 10.1111/mmi.13843.
  • Fukumura T, Makino F, Dietsche T, et al.
    Assembly and stoichiometry of the core structure of the bacterial flagellar type III export gate complex.
    PLOS Biology 15(8). e2002281(22pp) (2017) doi: 10.1371/journal.pbio.2002281
  • Kinoshita M, Aizawa S, Inoue Y, et al.
    The role of intrinsically disordered C-terminal region of FliK in substrate specificity switching of the bacterial flagellar type III export apparatus.
    Molecular Microbiology 105. 572-588 (2017) doi: 10.1111/mmi.13718
  • Hiraoka KD, Morimoto YV, Inoue Y, et al.
    Straight and rigid flagellar hook made by insertion of the FlgG specific sequence into FlgE.
    Scientific Reports 7. 46723(8pp) (2017) doi: 10.1038/srep46723.
  • Terahara N, Noguchi Y, Nakamura S, et al.
    Load- and polysaccharide-dependent activation of the Na+-type MotPS stator in the Bacillus subtilis flagellar motor.
    Scientific Reports 7. 46081(11pp) (2017) doi: 10.1038/srep46081
  • Renault TT, Abraham AO, Bergmiller T, et al.
    Bacterial flagella grow through an injection-diffusion mechanism.
    eLife, 6. e23136 (2017) doi: 10.7554/eLife.23136

All Publications