細胞は、分子から秩序構造を自己組織化する動的システムと捉えることが出来ます。小さな分子はどのような仕組みで「細胞」という広大な空間を認識し、適時適所で集合・離散しながら、細胞スケールの動的秩序や生命機能を生み出しているのでしょうか？私たちの研究チームは、主に細胞骨格をモデル系として用い、生細胞で観察される動的構造や機能が人工細胞で再現される条件を探るという構成論的手法で、この謎の解明に挑んでいます。分子の局所的な相互作用から、生命が成立する仕組みを紐解くことを目指します。
主要論文
Yamazaki Y, Miyata Y, Morigaki K, Miyazaki M.
Controlling Physical and Biochemical Parameters of Actin Nucleation Using a Patterned Model Lipid Membrane.
Nano Letters
24(6), 1825-1834 (2024)
doi: 10.1021/acs.nanolett.3c02742
Sakamoto R, Izri Z, Shimamoto Y, et al.
Geometric trade-off between contractile force and viscous drag determines the actomyosin-based motility of a cell-sized droplet.
Proceedings of the National Academy of Sciences of the United States of America
119(30), e2121147119 (2022)
doi: 10.1073/pnas.2121147119
Sakamoto R, Tanabe M, Hiraiwa T, et al.
Tug-of-war between actomyosin-driven antagonistic forces determines the positioning symmetry in cell-sized confinement.
Nature Communications
11(1), 3063 (2020)
doi: 10.1038/s41467-020-16677-9
Kubota H, Miyazaki M, Ogawa T, et al.
Processive Nanostepping of Formin mDia1 Loosely Coupled with Actin Polymerization.
Nano Letters
18(10), 6617-6624 (2018)
doi: 10.1021/acs.nanolett.8b03277
Kubota H, Miyazaki M, Ogawa T, et al.
Biphasic Effect of Profilin Impacts the Formin mDia1 Force-Sensing Mechanism in Actin Polymerization.
Biophysical Journal
113(2), 461-471 (2017)
doi: 10.1016/j.bpj.2017.06.012
Suzuki K, Miyazaki M, Takagi J, et al.
Spatial confinement of active microtubule networks induces large-scale rotational cytoplasmic flow.
Proceedings of the National Academy of Sciences of the United States of America
114(11), 2922-2927 (2017)
doi: 10.1073/pnas.1616001114
Miyazaki M, Chiba M, Eguchi H, et al.
Cell-sized spherical confinement induces the spontaneous formation of contractile actomyosin rings in vitro.
Nature Cell Biology
17(4), 480-489 (2015)
doi: 10.1038/ncb3142
Chiba M, Miyazaki M, Ishiwata S.
Quantitative analysis of the lamellarity of giant liposomes prepared by the inverted emulsion method.
Biophysical Journal
107(2), 346-354 (2014)
doi: 10.1016/j.bpj.2014.05.039
