Laboratory for Integrated Biodevice

Research Summary

This laboratory aims to promote integration and sophistication of biological experiments using micro structures and channels based on semiconductor fabrication methods. These sizes are near the size of hair or mosquito needle width (10～100 μm) or less. By exploiting these devices to chemical or biological fields, precise cell or molecular handling can be realized. Furthermore, our group focuses on unique functions of cells or tissue of living creatures. By combining this with microfabrication technology, we aim to new functional devices and propose future ideal machine models.

Research Theme

• Integrated and sophisticated biological experiments
• Novel micro- and nano-size biological devices

Main Publications List

• Ota N, Kanda GN, Moriguchi H, et al.
  A Microfluidic Platform Based on Robust Gas and Liquid Exchange for Long-Term Culturing of Explanted Tissues.
  Analytical Sciences (2019) doi: 10.2116/analsci.19P099
• Kawai T, Ota N, Okada K, et al.
  Ultrasensitive single cell metabolomics by capillary electrophoresis–mass spectrometry with a thin-walled tapered emitter and large-volume dual sample preconcentration.
  Analytical Chemistry 91, 10564–10572 (2019) doi: 10.1021/acs.analchem.9b01578
• Ota N, Yonamine Y, Asai T, et al.
  Isolating Single Euglena gracilis Cells by Glass Microfluidics for Raman Analysis of Paramylon Biogenesis.
  Analytical chemistry doi: 10.1021/acs.analchem.9b01007
• Tanaka Y, Funano SI, Noguchi Y, et al.
  A valve powered by earthworm muscle with both electrical and 100% chemical control.
  Scientific reports 9(1). 8042 (2019) doi: 10.1038/s41598-019-44116-3
• Tanaka N, Kogo T, Hirai N, et al.
  In-situ detection based on the biofilm hydrophilicity for environmental biofilm formation.
  Scientific reports doi: 10.1038/s41598-019-44167-6
• Tanaka N, Yamashita T, Yalikun Y, et al.
  An ultra-small fluid oscillation unit for pumping driven by self-organized three-dimensional bridging of pulsatile cardiomyocytes on elastic micro-piers.
  Sensors and Actuators B: Chemical 293. 256-264 (2019) doi: 10.1016/j.snb.2019.04.087
• Shen Y, Yalikun Y, Tanaka Y.
  Recent advances in microfluidic cell sorting systems
  Sensors and Actuators B: Chemical 282. 268-281 (2019) doi: 10.1016/j.snb.2018.11.025
• Ota N, Yalikun Y, Tanaka N, et al.
  Simple Isolation of Single Cell: Thin Glass Microfluidic Device for Observation of Isolated Single Euglena gracilis Cells.
  Analytical sciences (2019) doi: 10.2116/analsci.18P568
• Ota N, Yalikun Y, Suzuki T, et al.
  Enhancement in acoustic focusing of micro and nanoparticles by thinning a microfluidic device.
  Royal Society Open Science 6. 181776 (2019) doi: 10.1098/rsos.181776
• Funano SI, Ota N, Sato A, Tanaka Y.
  A method of packaging molecule/cell-patterns in an open space into a glass microfluidic channel by combining pressure-based low/room temperature bonding and fluorosilane patterning.
  Chemical Communications 53. 11193-11196 (2017) doi: 10.1039/c7cc04744d
• Yalikun Y, Tanaka Y.
  Ultra-thin glass sheet integrated transparent diaphragm pressure transducer.
  Sensors and Actuators A: Physical 263. 102-112 (2017) doi: 10.1016/j.sna.2017.05.047
• Tanaka N, Yamashita T, Sato A, et al.
  Simple agarose micro-confinement array and machine-learning-based classification for analyzing the patterned differentiation of mesenchymal stem cells.
  Plos One 12(4). e0173647 (2017) doi: 10.1371/journal.pone.0173647
• Tanaka Y, Noguchi Y, Yalikun Y, Kamamichi N.
  Earthworm muscle driven bio-micropump.
  Sensors and Actuators B-Chemical 242. 1186-1192 (2017) doi:10.1016/j.snb.2016.09.123
• Tanaka Y, Funano SI, Nishizawa Y, et al.
  An electric generator using living Torpedo electric organs controlled by fluid pressure-based alternative nervous systems.
  Scientific Reports 6. 25899 (2016) doi: 10.1038/srep25899
• Funano S, Tanaka N, Tanaka Y.
  Vapor-based micro/nano-partitioning of fluoro-functional group immobilization for long-term stable cell patterning.
  Rsc Advances 6(98). 96306-96313 (2016) doi: 10.1039/c6ra16906f
• Tanaka N, Moriguchi H, Sato A, et al.
  Microcasting with agarose gel via degassed polydimethylsiloxane molds for repellency-guided cell patterning.
  Rsc Advances 6(60). 54754-54762 (2016) doi: 10.1039/c6ra11563b
• Yalikun Y, Hosokawa Y, Iino T, Tanaka Y.
  An all-glass 12 μm ultra-thin and flexible microfluidic chip fabricated by femtosecond laser processing.
  Lab on a Chip 16(13). 2427-2433 (2016) doi: 10.1039/c6lc00132g
• Tanaka Y, Fujita H.
  Fluid driving system for a micropump by differentiating iPS cells into cardiomyocytes on a tent-like structure.
  Sensors and Actuators B-Chemical 210. 267-272 (2015) doi: 10.1016/j.snb.2014.12.069
• Moriguchi H, Kawai T, Tanaka Y.
  Simple bilayer on-chip valves using reversible sealability of PDMS.
  Rsc Advances 5(7). 5237-5243 (2015) doi: 10.1039/c4ra10300a
• Tanaka Y.
  Electric actuating valves incorporated into an all glass-based microchip exploiting the flexibility of ultra thin glass.
  Rsc Advances 3(26). 10213-10220 (2013) doi: 10.1039/c3ra41218k

All Publications

• 2019/11/15 Research Detecting the metabolites of a single cell
• 2019/10/11 Research Analyzing the metabolites produced by single motile cells
• 2019/10/10 Research Brain tissue kept alive for weeks on an artificial membrane
• 2019/07/25 Research Toward micro-hearts: tiny pumps driven by self-organizing cardiomyocytes
• 2019/07/08 Research Cyborg-like microchip valve driven by earthworm muscle