ロゴマーク
研究

研究

BDRでは、様々な分野の研究者が協力して、より高い目標に向かって研究を進めています。

セミナー・シンポジウム

セミナー・イベント

BDRでは、ライフサイエンス分野の国際的な研究者を招いて、年1回のシンポジウムや定期的なセミナーを開催しています。

働く・学ぶ

働く・学ぶ

BDRでは、様々なバックグラウンドを持つ人々を受け入れ、オープンで協力的な研究環境の構築に努めています。

つながる・楽しむ

つながる・楽しむ

BDRでは、様々なメディアや活動を通じて、研究の魅力や意義を社会に発信しています。

ニュース

ニュース

最新の研究、イベント、研究者のインタビューなど、理研BDRの最新情報をお届けします。

BDRについて

BDRについて

理研の強みを生かし学際的なアプローチで生命の根源を探求し、社会の課題に応えます。

坂本 健作 | 非天然型アミノ酸技術研究チーム

業績一覧

An integrated computational pipeline for designing high-affinity nanobodies with expanded genetic codes. Padhi AK, Kumar A, Haruna KI, Sato H, Tamura H, Nagatoishi S, Tsumoto K, Yamaguchi A, Iraha F, Takahashi M, Sakamoto K, Zhang KYJ. Brief Bioinform. 2021 Aug 20:bbab338. doi: 10.1093/bib/bbab338.

Direct homophilic interaction of LAMP2A with the two-domain architecture revealed by site-directed photo-crosslinks and steric hindrances in mammalian cells. Terasawa K, Kato Y, Ikami Y, Sakamoto K, Ohtake K, Kusano S, Tomabechi Y, Kukimoto-Niino M, Shirouzu M, Guan JL, Kobayashi T, Iwata T, Watabe T, Yokoyama S, Hara-Yokoyama M. Autophagy. 2021 Apr 14:1-19. doi: 10.1080/15548627.2021.1911017.

An expanded genetic code facilitates antibody chemical conjugation involving the lambda light chain. Kato A, Ohtake K, Tanaka Y, Yokoyama S, Sakamoto K, Shiraishi Y. Biochem Biophys Res Commun. 2021 Mar 26;546:35-39. doi: 10.1016/j.bbrc.2021.02.005.

Incorporation of Halogenated Amino Acids into Antibody Fragments at Multiple Specific Sites Enhances Antigen Binding. Hayashi A, Haruna KI, Sato H, Ito K, Makino C, Ito T, Sakamoto K. Chembiochem. 2021 Jan 5;22(1):120-123. doi: 10.1002/cbic.202000429.

Fully Productive Cell-Free Genetic Code Expansion by Structure-Based Engineering of Methanomethylophilus alvus Pyrrolysyl-tRNA Synthetase. Seki E, Yanagisawa T, Kuratani M, Sakamoto K, Yokoyama S. ACS Synth Biol. 2020 Apr 17;9(4):718-732. doi: 10.1021/acssynbio.9b00288.

Structural Basis for Genetic-Code Expansion with Bulky Lysine Derivatives by an Engineered Pyrrolysyl-tRNA Synthetase. Yanagisawa T, Kuratani M, Seki E, Hino N, Sakamoto K, Yokoyama S. Cell Chem Biol. 2019 Jul 18;26(7):936-949.e13. doi: 10.1016/j.chembiol.2019.03.008.

Cell-Free Protein Synthesis Using S30 Extracts from Escherichia coli RFzero Strains for Efficient Incorporation of Non-Natural Amino Acids into Proteins. Adachi J, Katsura K, Seki E, Takemoto C, Shirouzu M, Terada T, Mukai T, Sakamoto K, Yokoyama S.Int J Mol Sci. 2019 Jan 24;20(3). pii: E492. doi:10.3390/ijms20030492.

Synthetic Tyrosine tRNA Molecules with Noncanonical Secondary Structures. Sakamoto K, Hayashi A. Int J Mol Sci. 2018 Dec 26;20(1). pii: E92. doi:10.3390/ijms20010092.

Variants of the industrially relevant protease KP-43 with suppressed activity under alkaline conditions developed using expanded genetic codes. Osamura T, Okuda M, Yamaguchi A, Ohtake K, Sakamoto K, Takimura Y. Biochem Biophys Rep. 2018, 17:93-96. doi: 10.1016/j.bbrep.2018.12.001. eCollection 2019 Mar.

Pyrrolysyl-tRNA Synthetase with a Unique Architecture Enhances the Availability of Lysine Derivatives in Synthetic Genetic Codes. Yamaguchi A, Iraha F, Ohtake K, Sakamoto K. Molecules. 2018 Sep 26;23(10). pii: E2460 doi:10.3390/molecules23102460

Engineering of Escherichia coli β-lactamase TEM-1variants showing higher activity under acidic conditions than at the neutral pH. Takahashi M, Sakamoto K. Biochem Biophys Res Commun. 2018 Oct 20;505(1):333-337. doi:10.1016/j.bbrc.2018.09.096.

JQ1 affects BRD2-dependent and independent transcription regulation without disrupting H4-hyperacetylated chromatin states. Handoko L, Kaczkowski B, Hon CC, Lizio M, Wakamori M, Matsuda T, Ito T, Jeyamohan P, Sato Y, Sakamoto K, Yokoyama S, Kimura H, Minoda A, Umehara T. Epigenetics. 2018;13(4):410-431. doi: 10.1080/15592294.2018.1469891.

Engineering an Automaturing Transglutaminase with Enhanced Thermostability by Genetic Code Expansion with Two Codon Reassignments. Ohtake K, Mukai T, Iraha F, Takahashi M, Haruna KI, Date M, Yokoyama K, Sakamoto K. ACS Synth. Biol. 2018 Sep 21;7(9):2170-2176. doi: 10.1021/acssynbio.8b00157.

Genetic Code Expansion of the Silkworm Bombyx mori to Functionalize Silk Fiber. Teramoto H, Amano Y, Iraha F, Kojima K, Ito T, Sakamoto K. ACS Synthetic Biology 2018, 7(3):801-806. doi: 10.1021/acssynbio.7b00437

A reproducible and scalable procedure for preparing bacterial extracts for cell-free protein synthesis. Katsura K, Matsuda T, Tomabechi Y, Yonemochi M, Hanada K, Ohsawa N, Sakamoto K, Takemoto C, Shirouzu M. The Journal of Biochemistry, mvx039, https://doi.org/10.1093/jb/mvx039 (2017)

Covalently capturing protein interactions in living cells by site-specific incorporation of photo-cross-linkable amino acids. Hino, N. and Sakamoto, K. in “Photoaffinity labeling for structural probing within protein” (eds. Hatanaka, Y. and Hashimoto, M.) Springer Japan KK (in press, due on Nov. 13, 2017)

Extensive Survey of Antibody Invariant Positions for Efficient Chemical Conjugation Using Expanded Genetic Codes. Kato A, Kuratani M, Yanagisawa T, Ohtake K, Hayashi A, Amano Y, Kimura K, Yokoyama S, Sakamoto K. Shiraishi Y. Bioconjug Chem. 2017 Aug 16;28(8):2099-2108. doi: 10.1021/acs.bioconjchem.7b00265.

Adenovirus vector-based incorporation of a photo-cross-linkable amino acid into proteins in human primary cells and cancerous cell lines. Kita A, Hino N, Higashi S, Hirota K, Narumi R, Adachi J, Takafuji K, Ishimoto K, Okada Y, Sakamoto K, Tomonaga T, Takashima S, Mizuguchi H, Doi T. Scientific Reports 6, Article number: 36946 doi:10.1038/srep36946 (2016)

Crystal structure of eukaryotic translation initiation factor 2B. Kazuhiro Kashiwagi, Mari Takahashi, Madoka Nishimoto, Takuya B. Hiyama, Toshiaki Higo, Takashi Umehara, Kensaku Sakamoto, Takuhiro Ito and Shigeyuki Yokoyama. Nature, 531(7592):122-5, (2016), doi: 10.1038/nature16991

Incorporation of a Doubly Functionalized Synthetic Amino Acid into Proteins for Creating Chemical and Light-Induced Conjugates. Yamaguchi A, Matsuda T, Ohtake K, Yanagisawa T, Yokoyama S, Fujiwara Y, Watanabe T, Hohsaka T, Sakamoto K Bioconjug Chem, 27(1), 198-206 (2016).

Reassignment of a rare sense codon to a non-canonical amino acid in Escherichia coli . Mukai T, Yamaguchi A, Ohtake K, Takahashi M, Hayashi A, Iraha F, Kira S, Yanagisawa T, Yokoyama S, Hoshi H, Kobayashi T, Sakamoto K.
Nucleic Acids Res
., doi: 10.1093/nar/gkv787, (2015).

Highly reproductive Escherichia coli cells with no specific assignment to the UAG codon. Mukai T, Hoshi H, Ohtake K, Takahashi M, Yamaguchi A, Hayashi A, Yokoyama S, Sakamoto K. Sci Rep , 5, 9699 (2015).

Protein stabilization utilizing a redefined codon. Ohtake K, Yamaguchi A, Mukai T, Kashimura H, Hirano N, Haruki M, Kohashi S, Yamagishi K, Murayama K, Tomabechi Y, Itagaki T, Akasaka R, Kawazoe M, Takemoto C, Shirouzu M, Yokoyama S, Sakamoto K. Sci Rep , 5, 9762 (2015).

Tetrameric interaction of the ectoenzyme CD38 on the cell surface enables its catalytic and raft-Association activities, Hara-Yokoyama, M., Kukimoto-Niino, M., Terasawa, K., Harumiya, S., Podyma-Inoue, K. A., Hino, N., Sakamoto, K., Itoh, S., Hashii, N., Hiruta, Y., Kawasaki, N., Mishima-Tsumagari, C., Kaitsu, Y., Matsumoto, T., Wakiyama, M., Shirouzu, M., Kasama, T., Takayanagi, H., Utsunomiya-Tate, N., Takatsu, K., Katada, T., Hirabayashi, Y., Yokoyama, S. and Yanagishita, M., Structure 20, 1585-1595, 2012

Structural basis of interleukin-5 dimer recognition by its α receptor, Kusano, S., Kukimoto-Niino, M., Hino, N., Ohsawa, N., Ikutani, M., Takaki, S., Sakamoto, K., Hara-Yokoyama, M., Shirouzu, M., Takatsu, K. and Yokoyama, S., Protein Sci. 21, 850-864 2012

Wide-range protein photo-crosslinking achieved by a genetically encoded N ε -(benzyloxycarbonyl)lysine derivative with a diazirinyl moiety, Yanagisawa, T., Hino, H., Iraha. F., Mukai, T., Sakamoto, K., Yokoyama, S., Mol. BioSyst . 8 , 1131-1135, 2012

Structural basis for extracellular interactions between calcitonin receptor-like receptor and receptor activity-modifying protein 2 for adrenomedullin-specific binding, Kusano, S., Kukimoto-Niino, M., Hino, N., Ohsawa, N., Okuda, KI., Sakamoto, K., Shirouzu, M., Shindo, T. and Yokoyama, S., Protein Sci., 21, 199-210, 2012

Site-specific incorporation of unnatural amino acids into proteins in mammalian cells, Hino, N., Sakamoto, K., Yokoyama, S., in Unnatural Amino Acids: Methods in Molecular Biology , Volume 794, Part 3, pp. 215-228, 2012

Genetic-code evolution for protein synthesis with non-natural amino acids, Mukai, T., Yanagisawa, T., Ohtake, K., Wakamori, M., Adachi, J., Hino, N., Sato, A., Kobayashi, T., Hayashi, A., Shirouzu, M., Umehara, T., Yokoyama, S., Sakamoto, K., Biochem. Biophys. Res. Commun . 411 , 757-761, 2011

Genetic incorporation of a photo-crosslinkable amino acid reveals novel protein complexes with GRB2 in mammalian cells, Hino, N., Oyama, M., Sato, A., Mukai, T., Iraha, F., Hayashi, A., Kozuka-Hata, H., Yamamoto, T., Yokoyama, S. & Sakamoto, K., J. Mol. Biol. 406 , 343-53, 2011

Site-specific incorporation of 4-iodo-l-phenylalanine through opal suppression, Kodama, K., Nakayama, H., Sakamoto, K., Fukuzawa, S., Kigawa, T., Yabuki, T., Kitabatake, M., Takio, K., Yokoyama, S., J. Biochem . 148 , 179-187, 2010

Genetic encoding of non-natural amino acids in Drosophila melanogaster Schneider 2 cells, Mukai, T., Wakiyama, M., Sakamoto, K., Yokoyama, S., Protein Sci . 19 , 440-448, 2010

Genetic encoding of 3-iodo-l-tyrosine in Escherichia coli for single-wavelength anomalous dispersion phasing in protein crystallography, Sakamoto, K., Murayama, K., Oki, K., Iraha, F., Kato-Murayama, M., Takahashi, M., Ohtake, K., Kobayashi, T., Kuramitsu, S., Shirouzu, M., Yokoyama S., Structure 17 , 335-344, 2009

Recognition of non-a-amino substrates by pyrrolysyl-tRNA synthetase, Kobayashi, T., Yanagisawa, T., Sakamoto, K., Yokoyama, S., J. Mol. Biol . 385 , 1352-1360, 2009

Multi-step engineering of pyrrolysyl-tRNA synthetase to genetically encode N e -(o-azidobenzyloxycarbonyl)lysine for site-specific protein modification, Yanagisawa, T., Ishii, R., Fukunaga, R., Kobayashi, T., Sakamoto, K., Yokoyama, S., Chem. Biol . 15 , 1187-1197, 2008

Adding l-lysine derivatives to the genetic code of mammalian cells with engineered pyrrolysyl-tRNA synthetases, Mukai, T., Kobayashi, T., Hino, N., Yanagisawa, T., Sakamoto, K., Yokoyama, S., Biochem. Biophys. Res. Commun . 371 , 818-822, 2008

Crystallographic study of a site-specifically cross-linked protein complex with a genetically incorporated photoreactive amino acid, Sato, S., Mimasu, S., Sato, A., Hino, N., Sakamoto, K., Umehara, T., Yokoyama, S., Biochemistry 50, 250-257, 2011

Site-specific functionalization of proteins by organopalladium reactions, Kodama, K., Fukuzawa, S., Nakayama, H., Sakamoto, K., Kigawa, T., Yabuki, T., Matsuda, N., Shirouzu, M., Takio, K., Yokoyama, S., Tachibana, K., Chembiochem 8, 232-238, 2007

A new protein engineering approach combining chemistry and biology, Part I; Site-specific incorporation of 4-iodo-L-phenylalanine in vitro by using misacylated suppressor tRNA(Phe), Kodama, K., Fukuzawa, S., Sakamoto, K., Nakayama, H., Kigawa, T., Yabuki, T., Matsuda, N., Shirouzu, M., Takio, K., Tachibana, K., and Yokoyama, S., Chembiochem. 7, 1577-1581, 2006

Cation-π interaction in triterpene biosynthesis revealed by incorporating unnatural amino acids into the catalytic site of a squalene cyclase, Morikubo, N., Fukuda, Y., Ohtake, K., Shinya, N., Kiga, D., Sakamoto, K., Asanuma, M., Hirota, H., Yokoyama, S., Hoshino, T., J. Am. Chem. Soc . 128 , 13184-13194, 2006

Translation of 'rare' Codons in a Cell-free Protein Synthesis System from Escherichia coli, Chumpolkulwong, N., Sakamoto, K., Hayashi, A., Iraha, F., Shinya, N., Matsuda, N., Kiga, D., Urushibata, A., Shirouzu, M., Oki, K., Kigawa, T., and Yokoyama, S., J. Struct. Funct. Genomics 7, 31-36, 2006

DNA polymerase programmed with a hairpin DNA incorporates a multiple-instruction architecture into molecular computing, Komiya, K., Sakamoto, K., Kameda, A., Yamamoto, M., Ohuchi, A., Kiga, D., Yokoyama, S., Hagiya, M., BioSystems 83 , 18-25, 2006

Regioselective carbon-carbon bond formation in proteins with palladium catalysis; new protein chemistry by organometallic chemistry, Kodama, K., Fukuzawa, S., Nakayama, H., Kigawa, T., Sakamoto, K., Yabuki, T., Matsuda, N., Shirouzu, M., Takio, K., Tachibana, K., Yokoyama, S., Chembiochem 7 , 134-139, 2006

Development of an in vivo computer based on Escherichia coli, Nakagawa, H., Sakamoto, K., Sakakibara, Y., Proceedings of DNA11: the 11 th International Workshop on DNA based computers (London, Ontario, Canada), pp.68-77, 2005

Hairpin-based state machine and conformational addressing: Design and experiment, Kameda, A., Yamamoto, M., Uejima, H., Hagiya, M., Sakamoto, K., Ohuchi, A., Natural Computing 4 , 103-126, 2005

Structural snapshots of the KMSKS loop rearrangement for amino acid activation by bacterial tyrosyl-tRNA synthetase, Kobayashi T, Takimura T, Sekine R, Vincent K, Kamata K, Sakamoto K, Nishimura S, Yokoyama S., J Mol. Biol. 346, 105-117, 2005

Structural basis of nonnatural amino acid recognition by an engineered aminoacyl-tRNA synthetase for genetic code expansion, Kobayashi T, Sakamoto K, Takimura T, Sekine R, Vincent K, Kamata K, Nishimura S, Yokoyama S., Proc. Natl. Acad. Sci. USA 102, 1366-1371, 2005

Toward “wet” implementation of genetic algorithm for protein engineering, Sakamoto, K., Yamamura, M., Someya, H., Lecture Note in Computer Science 3384 , 308-318, 2005

The Escherichia coli argU10 (Ts) Phenotype is caused by a reduction in the cellular level of the argU tRNA for the rare codons AGA and AGG, Sakamoto, K., Ishimaru, S., Kobayashi, T., Walker, J. R., Yokoyama, S., J. Bacteriol . 186 , 5899-5905, 2004

Conformational addressing using the hairpin structure of single-strand DNA, Kameda, A., Yamamoto, M., Uejima, H., Hagiya, M., Sakamoto, K., Ohuchi, A., Lecture Notes in Computer Science 2943 , 219-224, 2004

Branching DNA Machines Based on Transitions of Hairpin Structures, Kubota, M., Ohtake, K., Komiya, K., Sakamoto, K., Hagiya, M., Proceedings of the 2003 Congress on Evolutionary Computation (CEC'03) , p. 2542-2548, 2003

Structural basis for orthogonal tRNA specificities of tyrosyl-tRNA synthetases for genetic code expansion, Kobayashi T., Nureki O., Ishitani R., Yaremchuk A., Tukalo M., Cusack S., Sakamoto K., Yokoyama S., Nat. Struct. Biol. 10, 425-432, 2003

An engineered Escherichia coli tyrosyl-tRNA synthetase for site-specific incorporation of an unnatural amino acid into proteins in eukaryotic translation and its application in a wheat germ cell-free system, Kiga, D., Sakamoto, K., Kodama, K., Kigawa, T., Matsuda, T., Yabuki, T., Shirouzu, M., Harada, Y., Nakayama, H., Takio, K., Hasegawa, Y., Endo, Y., Hirao, I., Yokoyama, S., Proc. Natl. Acad. Sci. USA 99 , 9715-9720, 2002

Indolmycin resistance of Streptomyces coelicolor A3(2) by induced expression of one of its two tryptophanyl-tRNA synthetases, Kitabatake, M., Ali, K., Demain, A., Sakamoto, K., Yokoyama, S., Söll, D., J. Biol. Chem . 277 , 23882-23887, 2002

Solution structure of an RNA fragment with the P7/P9.0 region and the 3'-terminal guanosine of the tetrahymena group I intron. Kitamura A, Muto Y, Watanabe S, Kim I, Ito T, Nishiya Y, Sakamoto K, Ohtsuki T, Kawai G, Watanabe K, Hosono K, Takaku H, Katoh E, Yamazaki T, Inoue T, Yokoyama S. RNA , 8(4), 440-451 (2002).

Shifted positioning of the nucleotide residues of tRNA recognized by arginyl-tRNA synthetase from Escherichia coli , Kiga, D., Sakamoto, K., Sato, S., Hirao, I., Yokoyama, S., Eur. J. Biochem . 268 , 1-8, 2001

Successive state transitions with I/O interface by molecules, Komiya, K., Sakamoto, K., Gouzu, H., Yokoyama, S., Arita, M., Nishikawa, A., Hagiya, M., Lecture Notes in Computer Science 2054 , 17-26, 2001

Synthesis of N-labeled peptidyl AMP, Narita, H., Moriguchi, T., Seio, K., Sekine, M., Miyaguchi, H., Sakamoto, K., Yokoyama, S., Nucleosides Nucleotides Nucleic Acids 19 , 1993-2003, 2000

Molecular computation by DNA hairpin formation, Sakamoto, K., Gouzu, H., Komiya, K., Kiga, D., Yokoyama, S., Yokomori, T., Hagiya, M., Science 288 , 1223-1226, 2000

Improving sequence design for DNA computing, Arita, M., Nisikawa, A., Hagiya, M., Komiya, K., Gouzu, H., Sakamoto, K., Proceedings of the Genetic and Evolutionary Computation Conference (GECCO 2000) , p. 875-890, 2000

State transitions by molecules, Sakamoto, K., Kiga, D., Komiya, K., Gouzu, H., Yokoyama, S., Ikeda, S., Sugiyama, H., Hagiya, M., BioSystems 52 , 81-91, 1999

Towards parallel evaluation and learning of Boolean m-formulas with molecules, Hagiya, M., Arita, M., Kiga, D., Sakamoto, K., Yokoyama, S., DIMACS Series in Discrete Mathematics and Theoretical Computer Science 48 , 57-72, 1999

RNA aptamers that specifically bind to the Ras-binding domain of Raf-1, Kimoto, M., Sakamoto, K., Shirouzu, M., Hirao, I., Yokoyama, S., FEBS Lett . 441 , 322-326, 1998

An RNA aptamer to the xanthine/guanine base with a distictive mode of purine recognition, Kiga, D., Futamura, Y., Sakamoto, K., and Yokoyama, S., Nucleic Acids Res. 26 , 1755-1760, 1998

Chemical synthesis and properties of conformationally fixed diuridine monophosphates as building blocks of the RNA turn motif, Seio, K., Wada, T., Sakamoto, K., Yokoyama, S., Sekine, M., Journal of Organic Chemistry 63 , 1429-1443, 1998

Cleavage Effect of oligoribonucleotides substituted at the cleavage sites with modified pyrimidine- and purine-nucleosides, Hosono K, Gozu H, Hosaka H, Sakamoto K, Yokoyama S, Takai K, Takaku H., Biochim. Biophys. Acta 1354 , 211-218, 1997

Study on site specific cleavage of RNA, Shirakura, H., Hosono, K., Kawai, G., Takai, K., Ohtsuki, T., Watanabe, K., Sakamoto, K., Yokoyama, S., Takaku H., Nucleic Acids Symposium Series 37 , 217-218, 1997

Synthesis and properties of conformationally rigid cyclouridylic acids having convalent bonding linkers between the uracil 5-position and the 5’-phosphate group, Seio, K., Kurasawa, O., Wada, T., Sakamoto, K., Yokoyama, S., Sekine, M., Nuclosides Nucleotides 16 , 1023-1032, 1997

An antibiotic-binding motif of an RNA fragment derived from the A-site-related region of Escherichia coli 16S rRNA. Miyaguchi H, Narita H, Sakamoto K, Yokoyama S. Nucleic Acids Res , 24(19), 3700-3706 (1996).

NMR studies of the effects of the 5'-phosphate group on conformational properties of 5-methylaminomethyluridine found in the first position of the anticodon of Escherichia coli tRNA 4 Arg , Sakamoto, K., Kawai, G., bnWatanabe, S., Niimi, T., Hayashi, N., Muto, Y., Watanabe, K., Satoh, T., Sekine, M., Yamaizumi, Z., Nishimura, S., Miyazawa, T., and Yokoyama, S., Biochemistry 35 , 6533-6538, 1996

Major identity determinants in the "augmented D helix" of tRNA Glu from Escherichia coli , Sekine, S., Nureki, O., Sakamoto, K., Niimi, T., Tateno, M., Go, M., Kohno, T., Brisson, A., Lapointe, J., Yokoyama, S., J. Mol. Biol . 256 , 685-700, 1996

Properties of RNAs containing modified bases at cleavage sites, Gozu, H., Hosono, K., Hosaka, H., Sakamoto, K., Yokoyama, S., Takai, K., Takaku, H., Nucleic Acids Symposium Series 35 , 201-202, 1996

Sequence-specific cleavage of oligoribonucleotide capable of forming a stem and loop structure, Hosaka, H., Sakabe, I., Sakamoto, K., Yokoyama, S., Takaku, H., J. Biol. Chem . 296 , 20090-20094, 1994

Characterization of the secondary structure of an oligonucleotide corresponding to the autocleavage site of a precursor RNA from bacteriophage T4, Hosaka, H., Sakabe, I., Sakamoto, K., Niimi, T., Yokoyama, S., Takaku H., Biochim. Biophys. Acta 1218 , 351-356, 1994

Chemical synthesis and properties of an interresidually cyclized uridylyl(3'-5')-uridine as a model of tRNA U turn structure having a sharp bend, Sekine, M., Seio, K., Wada, T., Sakamoto, K., Yokoyama, S., J. Am. Chem. Soc . 116 , 4469-4470, 1994

Mechanisms of the inhibition of reverse transcription by unmodified and modified antisense oligonucleotides, Hatta, T., Kim, S.-G., Nakashima, H., Yamamoto, N., Sakamoto, K., Yokoyama, S., Takaku, H., FEBS Lett . 330 , 161-164, 1993

A modified uridine in the first position of the anticodon of a minor species of arginine tRNA, the argU gene product, from Escherichia coli , Sakamoto, K., Kawai, G., Niimi, T., Satoh, T., Sekine, M., Yamaizumi, Z., Nishimura, S., Miyazawa, T., Yokoyama, S., Eur. J. Biochem . 216 , 369-375, 1993

Chemical modification and mutagenesis studies on zinc binding of aminoacyl-tRNA synthtase, Nureki, O., Kohno, T., Sakamoto, K., Miyazawa, T., and Yokoyama, S., J. Biol. Chem . 268 , 15368-15373, 1993

Synthesis of uridylyl(3'-5')uridine derivatives containing 5-(methylamino-methyl)uridine as a modified nucleoside found from E. coli tRNA Arg , Sekine, M., Seio, K., Satoh, T., Sakamoto, K., Yokoyama, S., Nucleosides Nucleotides 12 , 305-321, 1993

Cleavage reaction of a synthetic oligoribonucleotide corresponding to the autocleavage site of a precursor RNA from bacteriophage T4, Hosaka, H., Ogawa, T., Sakamoto, K., Yokoyama, S., Takaku, H., FEBS Lett . 293 , 204-206, 1991

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