Vascular niche and reprogramming for skeletal muscle stem cell
2019年3月22日 16:00 - 17:30
DB Bldg. SeminarRoom A7F
Stem Cell Institute, Paul & Sheila Wellstone Muscular Dystrophy Center, Department of Neurology, University of Minnesota Medical School
Muscle satellite cells are a stem cell population responsible for skeletal muscle growth, repair and regeneration. Maintenance of the balance between satellite cell differentiation and self-renewal is required for muscle homeostasis. By utilizing fluorescent reporter mice and muscle tissue clearing to investigate the specific niche for muscle satellite cells in 3 dimensions, we recently reported that the juxtavascular niche of satellite cells for stem cell maintenance via VEGF and Notch pathways (Verma, et al. Cell Stem Cell, 2018). Increased vascular density could induce increased number of satellite cells, which leads to a beneficial effect on muscular dystrophy model mice as a novel therapeutic method of Duchenne muscular dystrophy (DMD). We also identified that ASCL4, a basic-Helix-Loop-Helix transcription factor, possesses an ability to induce myogenic program in pluripotent stem cells, indicating that ASCL4 acts as a myogenic transcription factor during muscle development, and that ASCL4-mediated myogenic conversion of pluripotent stem cells will be a new therapy for DMD.