BDR researchers coming from diverse research fields are working together to achieve higher goals.

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Seminars & Symposia

BDR hosts annual symposium and regular seminars inviting international scientists in life science.

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Careers & Study

BDR embraces people from diverse backgrounds, and strives to create an open and supportive setting for research.



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About Us

About Us

Exploring the scientific foundations of life through interdisciplinary approaches to address society’s problems.

上田 昌宏チームリーダーの写真

Team Leader
Masahiro Ueda Ph.D.

Laboratory for Cell Signaling Dynamics

LocationOsaka / Osaka University BioSystems Building


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Toward a molecular understanding of cellular spontaneity

Our group studies how signal transduction function of living cells arises spontaneously from the dynamics of intracellular reaction networks (design principles), and the mechanisms that allow these systems to process signals under the strong influence of thermal and stochastic noise (computation principles). As a typical example of stochastic biomolecular computation in living cells, we specifically focus on the gradient sensing and directional cell migration seen in the cellular slime mold Dictyostelium discoideum. In this research project, by combining advanced imaging techniques including single-molecule detection with theoretical modeling, we aim to better understand these two principles while at the same time develop the techniques necessary to do so.

Research Theme

  • Spontaneous signal generation in living motile cells
  • Stochastic signal transduction and processing
  • Automated single-molecule imaging analysis in living cells
  • Organized randomness in hierarchical structure of biological systems

Selected Publications

Miyagawa T, Koteishi H, Kamimura Y, et al.
Structural basis of Gip1 for cytosolic sequestration of G protein in wide-range chemotaxis.
Nature Communications 9, 4635 (2018) doi: 10.1038/s41467-018-07035-x

Matsuoka S, Ueda M.
Mutual inhibition between PTEN and PIP3 generates bistability for polarity in motile cells.
Nature communications 9(1), 4481 (2018) doi: 10.1038/s41467-018-06856-0

Yasui M, Hiroshima M, Kozuka J, et al.
Automated single-molecule imaging in living cells.
Nature Communications 9, 3061 (2018) doi: 10.1038/s41467-018-05524-7

Yanagawa M, Hiroshima M, Togashi Y, et al.
Single-molecule diffusion-based estimation of ligand effects on G protein-coupled receptors.
Science signaling 11(548), eaao1917 (2018) doi: 10.1126/scisignal.aao1917

Kamimura Y, Miyanaga Y, Ueda M.
Heterotrimeric G-protein shuttling via Gip1 extends the dynamic range of eukaryotic chemotaxis.
Proceedings of the National Academy of Sciences of the United States of America 113(16), 4356-4361 (2016) doi: 10.1073/pnas.1516767113

Komatsuzaki A, Ohyanagi T, Tsukasaki Y, et al.
Compact Halo-ligand-conjugated quantum dots for multicolored single-molecule imaging of overcrowding GPCR proteins on cell membranes.
Small 11(12), 1396-1401 (2015) doi: 10.1002/smll.201402508

Yasui M, Matsuoka S, Ueda M.
PTEN Hopping on the Cell Membrane Is Regulated via a Positively-Charged C2 Domain.
Plos Computational Biology 10(9), e1003817 (2014) doi: 10.1371/journal.pcbi.1003817

Cai H, Katoh-Kurasawa M, Muramoto T, et al.
Nucleocytoplasmic Shuttling of a GATA Transcription Factor Functions as a Development Timer.
Science 343(6177), 1329-+ (2014) doi: 10.1126/science.1249531

Nishikawa M, Horning M, Ueda M, Shibata T.
Excitable Signal Transduction Induces Both Spontaneous and Directional Cell Asymmetries in the Phosphatidylinositol Lipid Signaling System for Eukaryotic Chemotaxis.
Biophysical Journal 106(3), 723-734 (2014) doi: 10.1016/j.bpj.2013.12.023

Okamoto M, Namba T, Shinoda T, et al.
TAG-1-assisted progenitor elongation streamlines nuclear migration to optimize subapical crowding.
Nature Neuroscience 16(11), 1556-1566 (2013) doi: 10.1038/nn.3525

Matsuoka S, Shibata T, Ueda M.
Asymmetric PTEN Distribution Regulated by Spatial Heterogeneity in Membrane-Binding State Transitions.
Plos Computational Biology 9(1), e1002862 (2013) doi: 10.1371/journal.pcbi.1002862

Nishimura SI, Ueda M, Sasai M.
Non-Brownian dynamics and strategy of amoeboid cell locomotion.
Physical Review E 85(4), 041909 (2012) doi: 10.1103/PhysRevE.85.041909

Tsujioka M, Yumura S, Inouye K, et al.
Talin couples the actomyosin cortex to the plasma membrane during rear retraction and cytokinesis.
Proceedings of the National Academy of Sciences of the United States of America 109(32), 12992-12997 (2012) doi: 10.1073/pnas.1208296109

Arai Y, Shibata T, Matsuoka S, et al.
Self-organization of the phosphatidylinositol lipids signaling system for random cell migration.
Proceedings of the National Academy of Sciences of the United States of America 107(27), 12399-12404 (2010) doi: 10.1073/pnas.0908278107