Laboratory for Mouse Genetic Engineering
[Closed Sep. 2023]
We are developing new techniques in mouse genetic engineering including direct genome editing on fertilized eggs of mice and microdevice-based easy and precise aggregation methods to obtain complete ES cell derived chimeric mice. By using these tools such as Triple CRISPR or Tol2 transgenesis, we are producing various useful genetically engineered mice within a short period of time. We are also studying cis-regulatory mechanisms in non-coding regions by using both molecular evolutionary analysis and genome editing technology to elucidate developmental and evolutionary mechanisms of mammals. Our recent achievement is the triple CRISPR gene knockout method, enabling 100% knockout of first generation mice. By using this method, phenotypic analysis can be done within three months without further crossing and breeding.
- Developing new techniques in highly efficient direct genome editing on fertilized eggs of mice
- Developing new transgenesis techniques on fertilized eggs of mice
- Study of cis-regulatory function in non-coding region of the mammalian genome
- Study of evolutionary mechanisms for acquiring novel function