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Research

Research

BDR researchers coming from diverse research fields are working together to achieve higher goals.

Seminars & Symposia

Seminars & Symposia

BDR hosts annual symposium and regular seminars inviting international scientists in life science.

Careers & Study

Careers & Study

BDR embraces people from diverse backgrounds, and strives to create an open and supportive setting for research.

Outreach

Outreach

BDR communicates the appeal and significance of our research to society through the use of various media and activities.

News

News

From research, events, people and everything in between, find out what’s going on at RIKEN BDR.

About Us

About Us

Exploring the scientific foundations of life through interdisciplinary approaches to address society’s problems.

Photo of Team leder, Yuko Mimori-Kiyosue

Team Leader
Yuko Mimori-Kiyosue Ph.D.

Laboratory for Molecular and Cellular Dynamics

Location Kobe / Developmental Biology Buildings

E-mailyuko.kiyosue[at]riken.jp

Please replace [at] with @.

Biomolecular and cellular functions highlighted by leading-edge light microscopy technologies

It is the working capacity of individual cells in our body that ensures we remain healthy or causes disease. The activities of cells are expressed through signal transduction, infrastructure construction and their dynamic regulation in a spatiotemporal manner. We are exploring the molecular and cellular mechanisms underlying normal development and diseases by employing a 3D high-resolution live-imaging technology, “lattice light-sheet microscopy”, which provides cellular information with unprecedented accuracy and precision. Currently, progress to establish a new style of cell biology using a large quantity of multidimensional information from lattice light-sheet microscopy is ongoing.

Research Theme

  • Functional study of intracellular machinery driving life
  • Research on molecular mechanisms that maintain cellular integrity and homeostasis
  • Development of a quantitative cell phenotyping method using lattice light-sheet microscopy
Time lapse movie of EB1 GFP in living fibroblasts
Microtubule dynamics in a dividing cell in 3D
Actin dynamics in 3D

Selected Publications

Yamashita N, Morita M, Legant WR, et al.
Three-dimensional tracking of plus-tips by lattice light-sheet microscopy permits the quantification of microtubule growth trajectories within the mitotic apparatus.
Journal of Biomedical Optics 20(10), 101206 (2015) doi: 10.1117/1.JBO.20.10.101206

Chen BC, Legant WR, Wang K, et al.
Lattice light-sheet microscopy: imaging molecules to embryos at high spatiotemporal resolution.
Science 346(6208), 1257998 (2014) doi: 10.1126/science.1257998

Shimozawa T, Yamagata K, Kondo T, et al.
Improving spinning disk confocal microscopy by preventing pinhole cross-talk for intravital imaging.
Proceedings of the National Academy of Sciences of the United States of America 110(9), 3399-3404 (2013) doi: 10.1073/pnas.1216696110

Nakamura S, Grigoriev I, Nogi T, et al.
Dissecting the nanoscale distributions and functions of microtubule-end-binding proteins EB1 and ch-TOG in interphase HeLa cells.
PLOS ONE 7(12), e51442 (2012) doi: 10.1371/journal.pone.0051442

Mimori-Kiyosue Y.
Shaping microtubules into diverse patterns: molecular connections for setting up both ends.
Cytoskeleton (Hoboken) 68(11), 603-618 (2011) doi: 10.1002/cm.20540

Hotta A, Kawakatsu T, Nakatani T, et al.
Laminin-based cell adhesion anchors microtubule plus ends to the epithelial cell basal cortex through LL5α/β.
The Journal of Cell Biology 189(5), 901-917 (2010) doi: 10.1083/jcb.200910095

Mimori-Kiyosue Y, Grigoriev I, Lansbergen G, et al.
CLASP1 and CLASP2 bind to EB1 and regulate microtubule plus-end dynamics at the cell cortex.
The Journal of Cell Biology 168(1), 141-153 (2005) doi: 10.1083/jcb.200405094

Mimori-Kiyosue Y, Tsukita S.
Search-and-capture of microtubules through plus-end-binding proteins (+TIPs).
The Journal of Biochemistry 134(3), 321-326 (2003) doi: 10.1093/jb/mvg148

Mimori-Kiyosue Y, Shiina N, Tsukita S.
The dynamic behavior of the APC-binding protein EB1 on the distal ends of microtubules.
Current Biology 10(14), 865-868 (2000) doi: 10.1016/S0960-9822(00)00600-X

Mimori-Kiyosue Y, Shiina N, Tsukita S.
Adenomatous polyposis coli (APC) protein moves along microtubules and concentrates at their growing ends in epithelial cells.
The Journal of Cell Biology 148(3), 505-518 (2000) doi: 10.1083/jcb.148.3.505

Members

Yuko Kiyosue

Team Leader

Yoshihiro Kawasaki

Senior Scientist

Akiko Hayashi

Research Part-time Worker I

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