BDR researchers coming from diverse research fields are working together to achieve higher goals.

Seminars & Symposia

Seminars & Symposia

BDR hosts annual symposium and regular seminars inviting international scientists in life science.

Careers & Study

Careers & Study

BDR embraces people from diverse backgrounds, and strives to create an open and supportive setting for research.



BDR communicates the appeal and significance of our research to society through the use of various media and activities.



From research, events, people and everything in between, find out what’s going on at RIKEN BDR.

About Us

About Us

Exploring the scientific foundations of life through interdisciplinary approaches to address society’s problems.

清成 寛チームリーダーの写真

Team Leader
Hiroshi Kiyonari Ph.D.

Laboratory for Animal Resources and Genetic Engineering

LocationKobe / MI R&D Center Building


Please replace [at] with @.

- Harnessing Experimental Animals -
Towards Comprehensive Animal Resource Management, Development, and Technical Support

The major mission of the Animal Resource Development Unit is to integrate advanced reproductive and developmental biology technologies and environment for animal research experiments through innovative research in the development of novel experimental animal models and related technical support activities. We utilize our expertise, not only to manage the state-of-the-art SPF rodent facility at RIKEN Kobe Campus, but also to accommodate a variety of animal research needs, including animal material and resource support, technical training, and education for proper conduct of animal research. In recent years, we have established colonies of a Soricomorpha species, suncus (Suncus murinus), a metatherian species, gray short-tail opossum (Monodelphis domestica), and a reptilian species, gecko (Paroedura picta) for prospective distribution services of these new resources to the research community. In addition, in close cooperation with Genetic Engineering Team, we are facilitating joint projects to generate and distribute novel genetically engineered mice with domestic and international biomedical research community, as well as conducting original research projects in live imaging and analyses of early mouse development.

Research Theme

  • Advancement of technologies in reproductive biology
  • In vitro development of early mouse embryos

Selected Publications

Nishiyama C, Saito Y, Sakaguchi A, et al.
Prolonged Myocardial Regenerative Capacity in Neonatal Opossum.
Circulation 146(2), 125-139 (2022) doi: 10.1161/CIRCULATIONAHA.121.055269

Inada K, Hagihara M, Tsujimoto K, et al.
Plasticity of neural connections underlying oxytocin-mediated parental behaviors of male mice.
Neuron 110(12), 2009-2023 (2022) doi: 10.1016/j.neuron.2022.03.033

Deviatiiarov R, Ishikawa K, Gazizova G, et al.
Integrative transcription start site analysis and physiological phenotyping reveal torpor-specific expression program in mouse skeletal muscle.
Communications Biology 4(1), 1290 (2021) doi: 10.1038/s42003-021-02819-2

Kiyonari H, Kaneko M, Abe T, et al.
Targeted gene disruption in a marsupial, Monodelphis domestica, by CRISPR/Cas9 genome editing.
Current Biology 31(17), 3956-3963 (2021) doi: 10.1016/j.cub.2021.06.056

Hirata T, Tohsato Y, Itoga H, et al.
NeuroGT: A brain atlas of neurogenic tagging CreER drivers for birthdate-based classification and manipulation of mouse neurons.
Cell Reports Methods 1, 100012 (2021) doi: 10.1016/j.crmeth.2021.100012

Kiyokawa H, Yamaoka A, Matsuoka C, et al.
Airway basal stem cells reutilize the embryonic proliferation regulator, Tgfβ-Id2 axis, for tissue regeneration.
Developmental Cell 56(13), 1917-1929 (2021) doi: 10.1016/j.devcel.2021.05.016

Minegishi K, Rothé B, Komatsu KR, et al.
Fluid flow-induced left-right asymmetric decay of Dand5 mRNA in the mouse embryo requires a Bicc1-Ccr4 RNA degradation complex.
Nature Communications 12(1), 4071 (2021) doi: 10.1038/s41467-021-24295-2

Morita R, Sanzen N, Sasaki H, et al.
Tracing the origin of hair follicle stem cells.
Nature 594(7864), 547-552 (2021) doi: 10.1038/s41586-021-03638-5

Abe T, Inoue KI, Furuta Y, Kiyonari H.
Pronuclear Microinjection during S-Phase Increases the Efficiency of CRISPR-Cas9-Assisted Knockin of Large DNA Donors in Mouse Zygotes.
Cell Reports 31(7), 107653 (2020) doi: 10.1016/j.celrep.2020.107653

Kajikawa E, Horo U, Ide T, et al.
Nodal paralogues underlie distinct mechanisms for visceral left-right asymmetry in reptiles and mammals.
Nature ecology & evolution 4(2), 261-269 (2020) doi: 10.1038/s41559-019-1072-2

Kiyonari H, Kaneko M, Abe T, et al.
Dynamic organelle localization and cytoskeletal reorganization during preimplantation mouse embryo development revealed by live imaging of genetically encoded fluorescent fusion proteins.
Genesis. 57(2), e23277 (2019) doi: 10.1002/dvg.23277

Kime C, Kiyonari H, Ohtsuka S, et al.
Induced 2C Expression and Implantation-Competent Blastocyst-like Cysts from Primed Pluripotent Stem Cells.
Stem cell reports 13(3), 485-498 (2019) doi: 10.1016/j.stemcr.2019.07.011

Nakao H, Harada T, Nakao K, et al.
A possible aid in targeted insertion of large DNA elements by CRISPR/Cas in mouse zygotes.
Genesis. 54(2), 65-77 (2016) doi: 10.1002/dvg.22914

Yoshida M, Kajikawa E, Kurokawa D, et al.
Conserved and divergent expression patterns of markers of axial development in eutherian mammals.
Developmental Dynamics 245(1), 67-86 (2016) doi: 10.1002/dvdy.24352

Katayama A, Nakatsuka A, Eguchi J, et al.
Beneficial impact of Gpnmb and its significance as a biomarker in nonalcoholic steatohepatitis.
Scientific Reports 5, 16920 (2015) doi: 10.1038/srep16920

Hara Y, Tatsumi K, Yoshida M, et al.
Optimizing and benchmarking de novo transcriptome sequencing: from library preparation to assembly evaluation.
BMC Genomics 16(1), 977 (2015) doi: 10.1186/s12864-015-2007-1

Popov LM, Marceau CD, Starkl PM, et al.
The adherens junctions control susceptibility to Staphylococcus aureus α-toxin.
Proceedings of the National Academy of Sciences of the United States of America 112(46), 14337-14342 (2015) doi: 10.1073/pnas.1510265112

Nakatsu Y, Iwashita M, Sakoda H, et al.
Prolyl isomerase Pin1 negatively regulates AMP-activated protein kinase (AMPK) by associating with the CBS domain in the γ subunit.
The Journal of Biological Chemistry 290(40), 24255-24266 (2015) doi: 10.1074/jbc.M115.658559

Hirate Y, Hirahara S, Inoue K.
Potential biomarkers of fatigue identified by plasma metabolome analysis in rats.
Development, Growth & Differentiation 57(8), 544-556 (2015) doi: 10.1111/dgd.12235

Imai Y, Kobayashi Y, Inoshita T, et al.
The Parkinson's Disease-Associated Protein Kinase LRRK2 Modulates Notch Signaling through the Endosomal Pathway.
PLOS Genetics 16(5), 517-532 (2015) doi: 10.1371/journal.pgen.1005503

Cui YL, Toyoda H, Sako T, et al.
A voxel-based analysis of brain activity in high-order trigeminal pathway in the rat induced by cortical spreading depression.
Neuroimage 108, 17-22 (2015) doi: 10.1016/j.neuroimage.2014.12.047

Lee MS, Hwang KS, Oh HW, et al.
IFT46 plays an essential role in cilia development.
Developmental Biology 400(2), 248-257 (2015) doi: 10.1016/j.ydbio.2015.02.009


Hiroshi Kiyonari

Team Leader

Takaya Abe

Technical Scientist

Kenichi Inoue

Expert Technician

Mayo Shigeta

Expert Technician

Mari Kaneko

Technical Staff

Miho Sato

Technical Staff

Aki Shiraishi

Technical Staff

Tomoko Tokunaga

Technical Staff

Tomoko Tokuhara

Technical Staff

Arisa Tobe

Technical Staff

Kohei Nakano

Technical Staff

Miwako Nomura

Technical Staff

Kana Bando

Technical Staff

Michiko Higashikawa

Technical Staff

Daisuke Yamamoto

Technical Staff

Riko Yoshimi

Technical Staff

Megumi Watase

Technical Staff

Masayo Okugawa