Laboratory for Animal Resources and Genetic Engineering | RIKEN BDR

Laboratory for Animal Resources and Genetic Engineering

Unit Leader

Hiroshi KiyonariPh.D.

  • Location:Kobe / Developmental Biology Buildings
  • E-mail:hiroshi.kiyonari[at]riken.jpPlease replace [at] with @.
  • Lab Website

- Harnessing Experimental Animals - Towards Comprehensive Animal Resource Management, Development, and Technical Support

Research Summary

The major mission of the Animal Resource Development Unit is to integrate advanced reproductive and developmental biology technologies and environment for animal research experiments through innovative research in the development of novel experimental animal models and related technical support activities. We utilize our expertise, not only to manage the state-of-the-art SPF rodent facility at RIKEN Kobe Campus, but also to accommodate a variety of animal research needs, including animal material and resource support, technical training, and education for proper conduct of animal research. In recent years, we have established colonies of a Soricomorpha species, suncus (Suncus murinus), a metatherian species, gray short-tail opossum (Monodelphis domestica), and a reptilian species, gecko (Paroedura picta) for prospective distribution services of these new resources to the research community. In addition, in close cooperation with Genetic Engineering Team, we are facilitating joint projects to generate and distribute novel genetically engineered mice with domestic and international biomedical research community, as well as conducting original research projects in live imaging and analyses of early mouse development.

Research Theme

  • Advancement of technologies in reproductive biology
  • In vitro development of early mouse embryos

Main Publications List

  • Kime C, Kiyonari H, Ohtsuka S, et al.
    Induced 2C Expression and Implantation-Competent Blastocyst-like Cysts from Primed Pluripotent Stem Cells
    Stem Cell Reports (2019) doi: 10.1016/j.stemcr.2019.07.011
  • Kiyonari H, Kaneko M, Abe T, et al.
    Dynamic organelle localization and cytoskeletal reorganization during preimplantation mouse embryo development revealed by live imaging of genetically encoded fluorescent fusion proteins.
    Genesis. 57(2). e23277 (2019) doi: 10.1002/dvg.23277
  • Nakao H, Harada T, Nakao K, et al.
    A possible aid in targeted insertion of large DNA elements by CRISPR/Cas in mouse zygotes.
    Genesis. 54(2). 65-77 (2016) doi: 10.1002/dvg.22914
  • Yoshida M, Kajikawa E, Kurokawa D, et al.
    Conserved and divergent expression patterns of markers of axial development in eutherian mammals.
    Developmental Dynamics 245(1). 67-86 (2016) doi: 10.1002/dvdy.24352
  • Katayama A, Nakatsuka A, Eguchi J, et al.
    Beneficial impact of Gpnmb and its significance as a biomarker in nonalcoholic steatohepatitis.
    Scientific Reports 5, 16920 (2015). doi: 10.1038/srep16920
  • Hara Y, Tatsumi K, Yoshida M, et al.
    Optimizing and benchmarking de novo transcriptome sequencing: from library preparation to assembly evaluation.
    BMC Genomics 16(1). 977 (2015) doi: 10.1186/s12864-015-2007-1
  • Popov LM, Marceau CD, Starkl PM, et al.
    The adherens junctions control susceptibility to Staphylococcus aureus α-toxin.
    Proceedings of the National Academy of Sciences of the United States of America 112(46). 14337-14342 (2015) doi: 10.1073/pnas.1510265112
  • Nakatsu Y, Iwashita M, Sakoda H, et al.
    Prolyl isomerase Pin1 negatively regulates AMP-activated protein kinase (AMPK) by associating with the CBS domain in the γ subunit.
    The Journal of Biological Chemistry 290(40). 24255-24266 (2015) doi: 10.1074/jbc.M115.658559
  • Hirate Y, Hirahara S, Inoue K
    Potential biomarkers of fatigue identified by plasma metabolome analysis in rats.
    Development, Growth & Differentiation 57(8). 544-556 (2015) doi: 10.1111/dgd.12235
  • Imai Y, Kobayashi Y, Inoshita T, et al.
    The Parkinson's Disease-Associated Protein Kinase LRRK2 Modulates Notch Signaling through the Endosomal Pathway.
    PLOS Genetics 16(5), 517-532 (2015). doi: 10.1371/journal.pgen.1005503
  • Cui YL, Toyoda H, Sako T, et al.
    A voxel-based analysis of brain activity in high-order trigeminal pathway in the rat induced by cortical spreading depression.
    Neuroimage 108. 17-22 (2015) doi: 10.1016/j.neuroimage.2014.12.047
  • Lee MS, Hwang KS, Oh HW, et al.
    IFT46 plays an essential role in cilia development.
    Developmental Biology 400(2). 248-257 (2015) doi: 10.1016/j.ydbio.2015.02.009

Member

Hiroshi KiyonariUnit Leader hiroshi.kiyonari[at]riken.jp
Yasuhide FurutaResearch Scientist*
Takaya AbeResearch Scientist
Kenichi InoueTechinical Staff
Karin AKiyamaTechinical Staff
Mari KanekoTechinical Staff
Miho SatoTechinical Staff
Mayo ShigetaTechinical Staff
Aki ShiraishiTechinical Staff
Tomoko TokunagaTechinical Staff
Masako NodaTechinical Staff
Daisuke YamamotoTechinical Staff
Miwako NomuraTechinical Staff
Tomoko TokuharaTechinical Staff
Kana BandoTechinical Staff*
Megumi WataseTechinical Staff
Kohei NakanoTechinical Staff
Michiko HigashikawaTechinical Staff*
Masayo OkugawaAssistant

*:concurrent / Please replace [at] with @.