Laboratory for Animal Resources and Genetic Engineering
- Location：Kobe / Developmental Biology Buildings
- E-mail：hiroshi.kiyonari[at]riken.jpPlease replace [at] with @.
- Lab Website
- Harnessing Experimental Animals - Towards Comprehensive Animal Resource Management, Development, and Technical Support
The major mission of the Animal Resource Development Unit is to integrate advanced reproductive and developmental biology technologies and environment for animal research experiments through innovative research in the development of novel experimental animal models and related technical support activities. We utilize our expertise, not only to manage the state-of-the-art SPF rodent facility at RIKEN Kobe Campus, but also to accommodate a variety of animal research needs, including animal material and resource support, technical training, and education for proper conduct of animal research. In recent years, we have established colonies of a Soricomorpha species, suncus (Suncus murinus), a metatherian species, gray short-tail opossum (Monodelphis domestica), and a reptilian species, gecko (Paroedura picta) for prospective distribution services of these new resources to the research community. In addition, in close cooperation with Genetic Engineering Team, we are facilitating joint projects to generate and distribute novel genetically engineered mice with domestic and international biomedical research community, as well as conducting original research projects in live imaging and analyses of early mouse development.
- Advancement of technologies in reproductive biology
- In vitro development of early mouse embryos
Main Publications List
Abe T, Inoue K, Furuta Y, Kiyonari H.
Pronuclear Microinjection during S-phase Increases the Efficiency of CRISPR-Cas9-Assisted Knockin of Large DNA Donors in Mouse Zygotes
Cell Reports (2020) doi: 10.1016/j.celrep.2020.107653
Kajikawa E, Horo U, Ide T, et al.
Nodal paralogues underlie distinct mechanisms for visceral left-right asymmetry in reptiles and mammals
Nature Ecology & Evolution (2020) doi: 10.1038/s41559-019-1072-2
Kime C, Kiyonari H, Ohtsuka S, et al.
Induced 2C Expression and Implantation-Competent Blastocyst-like Cysts from Primed Pluripotent Stem Cells
Stem Cell Reports (2019) doi: 10.1016/j.stemcr.2019.07.011
Kiyonari H, Kaneko M, Abe T, et al.
Dynamic organelle localization and cytoskeletal reorganization during preimplantation mouse embryo development revealed by live imaging of genetically encoded fluorescent fusion proteins.
Genesis. 57(2). e23277 (2019) doi: 10.1002/dvg.23277
Nakao H, Harada T, Nakao K, et al.
A possible aid in targeted insertion of large DNA elements by CRISPR/Cas in mouse zygotes.
Genesis. 54(2). 65-77 (2016) doi: 10.1002/dvg.22914
Yoshida M, Kajikawa E, Kurokawa D, et al.
Conserved and divergent expression patterns of markers of axial development in eutherian mammals.
Developmental Dynamics 245(1). 67-86 (2016) doi: 10.1002/dvdy.24352
Katayama A, Nakatsuka A, Eguchi J, et al.
Beneficial impact of Gpnmb and its significance as a biomarker in nonalcoholic steatohepatitis.
Scientific Reports 5, 16920 (2015). doi: 10.1038/srep16920
Hara Y, Tatsumi K, Yoshida M, et al.
Optimizing and benchmarking de novo transcriptome sequencing: from library preparation to assembly evaluation.
BMC Genomics 16(1). 977 (2015) doi: 10.1186/s12864-015-2007-1
Popov LM, Marceau CD, Starkl PM, et al.
The adherens junctions control susceptibility to Staphylococcus aureus α-toxin.
Proceedings of the National Academy of Sciences of the United States of America 112(46). 14337-14342 (2015) doi: 10.1073/pnas.1510265112
Nakatsu Y, Iwashita M, Sakoda H, et al.
Prolyl isomerase Pin1 negatively regulates AMP-activated protein kinase (AMPK) by associating with the CBS domain in the γ subunit.
The Journal of Biological Chemistry 290(40). 24255-24266 (2015) doi: 10.1074/jbc.M115.658559
Hirate Y, Hirahara S, Inoue K
Potential biomarkers of fatigue identified by plasma metabolome analysis in rats.
Development, Growth & Differentiation 57(8). 544-556 (2015) doi: 10.1111/dgd.12235
Imai Y, Kobayashi Y, Inoshita T, et al.
The Parkinson's Disease-Associated Protein Kinase LRRK2 Modulates Notch Signaling through the Endosomal Pathway.
PLOS Genetics 16(5), 517-532 (2015). doi: 10.1371/journal.pgen.1005503
Cui YL, Toyoda H, Sako T, et al.
A voxel-based analysis of brain activity in high-order trigeminal pathway in the rat induced by cortical spreading depression.
Neuroimage 108. 17-22 (2015) doi: 10.1016/j.neuroimage.2014.12.047
Lee MS, Hwang KS, Oh HW, et al.
IFT46 plays an essential role in cilia development.
Developmental Biology 400(2). 248-257 (2015) doi: 10.1016/j.ydbio.2015.02.009
|Hiroshi KiyonariTeam Leader||hiroshi.kiyonari[at]riken.jp|
|Yasuhide FurutaResearch Scientist*|
|Takaya AbeResearch Scientist|
|Kenichi InoueTechinical Staff|
|Karin AKiyamaTechinical Staff|
|Mari KanekoTechinical Staff|
|Miho SatoTechinical Staff|
|Mayo ShigetaTechinical Staff|
|Aki ShiraishiTechinical Staff|
|Tomoko TokunagaTechinical Staff|
|Masako NodaTechinical Staff|
|Daisuke YamamotoTechinical Staff|
|Miwako NomuraTechinical Staff|
|Tomoko TokuharaTechinical Staff|
|Kana BandoTechinical Staff*|
|Megumi WataseTechinical Staff|
|Kohei NakanoTechinical Staff|
|Michiko HigashikawaTechinical Staff*|
*：concurrent / Please replace [at] with @.