Team Leader
Takashi Jin
D.Sci.
Laboratory for Nano-Bio Probes
[Closed Mar. 2023]
E-mailtjin[at]riken.jp
Please replace [at] with @.
Our research team develops novel nano-bio probes for visualization of molecular and cellular dynamics in biological systems. We have synthesized a variety of nano-bio probes such as single-molecule imaging probes using quantum dots, cellular environment sensitive probes, and cellular state controllable probes using external magnetic fields. Recently, we have developed deep-tissue imaging probes to visualize cellular dynamics in vivo. We synthesize multi-colored fluorescent probes that emit NIR light (1000-1400 nm; the second optical window in living tissues), using cyanine dyes, fluorescent proteins, quantum dots, and rare-earth ions. By using the NIR fluorescent probes, we will visualize cancer metastasis and immune reactions at the cellular level in living systems. We are also developing probe delivery techniques (using DDS) for targeting cancer tissues and organs in small animals.
Research Theme
- Multi-colored single-molecule imaging probes with high brightness and photo-stability
- Cellular-environment sensitive near-infrared probes that respond to membrane potential, pH, and solvent polarity
- Bioluminescence-coupled self illuminating near-infrared probes
- Near-infrared probes that can be used in the second optical window (1000-1500 nm)
- In vivo fluorescence imaging of cancer metastasis, immune/ inflammation responses at the cellular level
Selected Publications
Swamy MMM, Zubir MZM, Mutmainah, et al.
A near-infrared fluorescent long-chain fatty acid toward optical imaging of cardiac metabolism in living mice.
Analyst
147(19), 4206-4212 (2022)
doi: 10.1039/d2an00999d
Swamy MMM, Murai Y, Monde K, et al.
Shortwave-Infrared Fluorescent Molecular Imaging Probes Based on π-Conjugation Extended Indocyanine Green.
Bioconjugate Chemistry
32(8), 1541-1547 (2021)
doi: 10.1021/acs.bioconjchem.1c00253
Tsuboi S, Jin T.
Dual-colour (near-infrared/visible) emitting annexin V for fluorescence imaging of tumour cell apoptosis in vitro and in vivo.
RSC Advances
10, 38244-38250 (2020)
doi: 10.1039/d0ra06495e
Tiwari D.K, Tiwari M, Jin T.
Near-infrared fluorescent protein and bioluminescence-based probes for high-resolution in vivo optical imaging.
Materials Advances
1, 967-987 (2020)
doi: 10.1039/d0ma00273a
Tsuboi S, Jin T.
Shortwave-infrared (SWIR) fluorescence molecular imaging using indocyanine green-antibody conjugates for the optical diagnostics of cancerous tumours.
RSC Advances
10, 28171-28179 (2020)
doi: 10.1039/d0ra04710d
Tsuboi S, Jin T.
BRET-based dual-color (visible/near-infrared) molecular imaging using a quantum dot/EGFP-luciferase conjugate.
RSC Advances
9, 34964-34971 (2019)
doi: 10.1039/c9ra07011g
Jin T.
Recent progress in NIR fluorophores emitting over 1000 nm for bioimaging.
ECS Journal of Solid State Science and Technology
8, R9-R13 (2019)
doi: 10.1149/2.0111901jss
Tsuboi S, Jin T.
Fluorescent recombinant protein conjugated near-infrared emitting quantum dots for in vitro and in vivo dual-color molecular imaging.
ChemBioChem
20, 565-575 (2019)
doi: 10.1002/cbic.201800506
Tsuboi S, Jin T.
Recombinant Protein (Luciferase-IgG Binding Domain) Conjugated Quantum Dots for BRET-Coupled Near-Infrared Imaging of Epidermal Growth Factor Receptors.
Bioconjugate Chemistry
29(4), 1466-1574 (2018)
doi: 10.1021/acs.bioconjchem.8b00149
Tsuboi S, Sasaki A, Sakata T, Yasuda H et al.
Immunoglobulin binding (B1) domain mediated antibody conjugation to quantum dots for in vitro and in vivo molecular imaging.
Chemical Communications
53, 9450-9453 (2017)
doi: 10.1039/c7cc04966h
Tsuboi S, Jin T.
Bioluminescence Resonance Energy Transfer (BRET)-coupled Annexin V-functionalized Quantum Dots for Near-Infrared Optical Detection of Apoptotic Cells.
Chembiochem : a European journal of chemical biology
18(22), 2231-2235 (2017)
doi: 10.1002/cbic.201700486