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Research

Research

BDR researchers coming from diverse research fields are working together to achieve higher goals.

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BDR hosts annual symposium and regular seminars inviting international scientists in life science.

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About Us

About Us

Exploring the scientific foundations of life through interdisciplinary approaches to address society’s problems.

Photo of Team leder, Takashi Jin

Team Leader
Takashi Jin D.Sci.

Laboratory for Nano-Bio Probes

Location Osaka / Quantitative Biology Buildings

E-mailtjin[at]riken.jp

Please replace [at] with @.

Development of near-infrared bio probes for non-invasive molecular imaging

Our research team develops novel nano-bio probes for visualization of molecular and cellular dynamics in biological systems. We have synthesized a variety of nano-bio probes such as single-molecule imaging probes using quantum dots, cellular environment sensitive probes, and cellular state controllable probes using external magnetic fields. Recently, we have developed deep-tissue imaging probes to visualize cellular dynamics in vivo. We synthesize multi-colored fluorescent probes that emit NIR light (1000-1400 nm; the second optical window in living tissues), using cyanine dyes, fluorescent proteins, quantum dots, and rare-earth ions. By using the NIR fluorescent probes, we will visualize cancer metastasis and immune reactions at the cellular level in living systems. We are also developing probe delivery techniques (using DDS) for targeting cancer tissues and organs in small animals.

Research Theme

  • Multi-colored single-molecule imaging probes with high brightness and photo-stability
  • Cellular-environment sensitive near-infrared probes that respond to membrane potential, pH, and solvent polarity
  • Bioluminescence-coupled self illuminating near-infrared probes
  • Near-infrared probes that can be used in the second optical window (1000-1500 nm)
  • In vivo fluorescence imaging of cancer metastasis, immune/ inflammation responses at the cellular level

Selected Publications

Swamy MMM, Murai Y, Monde K, et al.
Shortwave-Infrared Fluorescent Molecular Imaging Probes Based on π-Conjugation Extended Indocyanine Green.
Bioconjugate Chemistry 32(8), 1541-1547 (2021) doi: 10.1021/acs.bioconjchem.1c00253

Tsuboi S, Jin T.
Dual-colour (near-infrared/visible) emitting annexin V for fluorescence imaging of tumour cell apoptosis in vitro and in vivo.
RSC Advances 10, 38244-38250 (2020) doi: 10.1039/d0ra06495e

Tiwari D.K, Tiwari M, Jin T.
Near-infrared fluorescent protein and bioluminescence-based probes for high-resolution in vivo optical imaging.
Materials Advances 1, 967-987 (2020) doi: 10.1039/d0ma00273a

Tsuboi S, Jin T.
Shortwave-infrared (SWIR) fluorescence molecular imaging using indocyanine green-antibody conjugates for the optical diagnostics of cancerous tumours.
RSC Advances 10, 28171-28179 (2020) doi: 10.1039/d0ra04710d

Tsuboi S, Jin T.
BRET-based dual-color (visible/near-infrared) molecular imaging using a quantum dot/EGFP-luciferase conjugate.
RSC Advances 9, 34964-34971 (2019) doi: 10.1039/c9ra07011g

Jin T.
Recent progress in NIR fluorophores emitting over 1000 nm for bioimaging.
ECS Journal of Solid State Science and Technology 8, R9-R13 (2019) doi: 10.1149/2.0111901jss

Tsuboi S, Jin T.
Fluorescent recombinant protein conjugated near-infrared emitting quantum dots for in vitro and in vivo dual-color molecular imaging.
ChemBioChem 20, 565-575 (2019) doi: 10.1002/cbic.201800506

Tsuboi S, Jin T.
Recombinant Protein (Luciferase-IgG Binding Domain) Conjugated Quantum Dots for BRET-Coupled Near-Infrared Imaging of Epidermal Growth Factor Receptors.
Bioconjugate Chemistry 29(4), 1466-1574 (2018) doi: 10.1021/acs.bioconjchem.8b00149

Tsuboi S, Sasaki A, Sakata T, Yasuda H et al.
Immunoglobulin binding (B1) domain mediated antibody conjugation to quantum dots for in vitro and in vivo molecular imaging.
Chemical Communications 53, 9450-9453 (2017) doi: 10.1039/c7cc04966h

Tsuboi S, Jin T.
Bioluminescence Resonance Energy Transfer (BRET)-coupled Annexin V-functionalized Quantum Dots for Near-Infrared Optical Detection of Apoptotic Cells.
Chembiochem : a European journal of chemical biology 18(22), 2231-2235 (2017) doi: 10.1002/cbic.201700486

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