Laboratory for Nano-Bio Probes | RIKEN BDR

Laboratory for Nano-Bio Probes

Team Leader

Takashi JinD.Sci.

  • Location:Osaka / Quantitative Biology Buildings
  • E-mail:tjin[at]riken.jpPlease replace [at] with @.

Development of near-infrared bio probes for non-invasive molecular imaging

Research Summary

Our research team develops novel nano-bio probes for visualization of molecular and cellular dynamics in biological systems. We have synthesized a variety of nano-bio probes such as single-molecule imaging probes using quantum dots, cellular environment sensitive probes, and cellular state controllable probes using external magnetic fields. Recently, we have developed deep-tissue imaging probes to visualize cellular dynamics in vivo. We synthesize multi-colored fluorescent probes that emit NIR light (1000-1400 nm; the second optical window in living tissues), using cyanine dyes, fluorescent proteins, quantum dots, and rare-earth ions. By using the NIR fluorescent probes, we will visualize cancer metastasis and immune reactions at the cellular level in living systems. We are also developing probe delivery techniques (using DDS) for targeting cancer tissues and organs in small animals.

Minute difference in size of Quantum dots produces different fluorescent colors

Cell surface proteins visualized in multi-color by Quantum dots

Research Theme

  • Multi-colored single-molecule imaging probes with high brightness and photo-stability
  • Cellular-environment sensitive near-infrared probes that respond to membrane potential, pH, and solvent polarity
  • Bioluminescence-coupled self illuminating near-infrared probes
  • Near-infrared probes that can be used in the second optical window (1000-1500 nm)
  • In vivo fluorescence imaging of cancer metastasis, immune/ inflammation responses at the cellular level

Main Publications List

  • Tsuboi S, Jin T.
    Fluorescent recombinant protein conjugated near-infrared emitting quantum dots for in vitro and in vivo dual-color molecular imaging.
    ChemBioChem (2018) doi: 10.1002/cbic.201800506
  • Komatsu N, Terai K, Imanishi A, Kamioka Y, et al.
    A platform of BRET-FRET hybrid biosensors for optogenetics, chemical screening, and in vivo imaging.
    Scientific Reports 8. 8984 (2018) doi: 10.1038/s41598-018-27174-x
  • Tsuboi S, Jin T.
    Recombinant Protein (Luciferase-IgG Binding Domain) Conjugated Quantum Dots for BRET-Coupled Near-Infrared Imaging of Epidermal Growth Factor Receptors.
    Bioconjugate Chemistry 29(4). 1466-1574 (2018) doi: 10.1021/acs.bioconjchem.8b00149
  • Tsuboi S, Yamada S, Nakane Y, Sakata T, et al.
    Water-soluble near-infrared fluorophores emitting over 1000 nm and their application to in vivo imaging in the second optical window (1000-1400nm)
    ECS Journal of Solid State Science and Technology 7(1). 3093-3101 (2018) doi: 10.1149/2.0141801jss
  • Zamberlan F, Turyanska L, Patanè A, Liu Z, et al.
    Stable DHLA–PEG capped PbS quantum dots: from synthesis to near-infrared biomedical imaging.
    Journal of Materials Chemistry B 6. 550-555(2018) doi: 10.1039/C7TB02912H
  • Morishita Y, Hironaka K, Lee SW, Jin T et al.
    Reconstructing 3D deformation dynamics for curved epithelial sheet morphogenesis from positional data of sparsely-labeled cells.
    Nature Communications 8(15). (2017) doi:10.1038/s41467-017-00023-7
  • Oura M, Yamamoto J, Jin T and Kinjo M.
    Investigation of pH-dependent photophysical properties of quantum nanocrystals by fluorescence correlation spectroscopy.
    Optics Express 25.1435-1443(2017) doi: 10.1364/OE.25.001435
  • Tsuboi S, Sasaki A, Sakata T, Yasuda H et al.
    Immunoglobulin binding (B1) domain mediated antibody conjugation to quantum dots for in vitro and in vivo molecular imaging.
    Chemical Communications 53. 9450-9453 (2017) doi: 10.1039/c7cc04966h
  • Tsuboi S and Jin T.
    Bioluminescence Resonance Energy Transfer (BRET)-coupled Annexin V-functionalized Quantum Dots for Near-Infrared Optical Detection of Apoptotic Cells.
    ChemBioChem 18(22).2231-2235(2017) doi: 10.1002/cbic.201700486
  • Jin T, Tsuboi S, Komatsuzaki A, Imamura Y, et al.
    Enhancement of aqueous stability and fluorescence brightness of indocyanine green using small calix[4]arene micelles for near-infrared fluorescence imaging.
    Medical Chemical Communications 7. 623-631(2016) doi: 10.1039/C5MD00580A