Team Leader
Takuhiro Ito
Ph.D.
Laboratory for Translation Structural Biology
LocationYokohama
E-mailtakuhiro.ito[at]riken.jp
Please replace [at] with @.
We study three dimensional structures of the translation factors and/or the ribosome, mainly by X-ray crystallography and cryo-EM. We focus on the structural analysis of the large complexes that function in the eukaryotic translational system, in order to reveal the complicated mechanisms of eukaryotic translation.
Research Theme
- Structural biology on translational system in eukaryotes
- Development of the sample preparation methods suitable for the crystallization/cryo-EM analysis of macromolecular complexes
Selected Publications
Tanaka M, Yokoyama T, Saito H, et al.
Boric acid intercepts 80S ribosome migration from AUG-stop by stabilizing eRF1.
Nature Chemical Biology
20, 605-614 (2024)
doi: 10.1038/s41589-023-01513-0
Matsuura-Suzuki E, Shimazu T, Takahashi M, et al.
METTL18-mediated histidine methylation of RPL3 modulates translation elongation for proteostasis maintenance.
eLife
11, e72780 (2022)
doi: 10.7554/eLife.72780
Kashiwagi K, Shichino Y, Osaki T, et al.
eIF2B-capturing viral protein NSs suppresses the integrated stress response.
Nature Communications
12, 7102 (2021)
doi: 10.1038/s41467-021-27337-x
Zyryanova AF, Kashiwagi K, Rato C, et al.
ISRIB blunts the integrated stress response by allosterically antagonising the inhibitory effect of phosphorylated eIF2 on eIF2B.
Molecular Cell
81, 88-103 (2021)
doi: 10.1016/j.molcel.2020.10.031
Yokoyama T, Machida K, Iwasaki W, et al.
HCV IRES Captures an Actively Translating 80S Ribosome.
Molecular Cell
74, 1205-1214 (2019)
doi: 10.1016/j.molcel.2019.04.022
Kashiwagi K, Yokoyama T, Nishimoto M, et al.
Structural basis for eIF2B inhibition in integrated stress response.
Science
364, 495-499 (2019)
doi: 10.1126/science.aaw4104
Iwasaki S, Iwasaki W, Takahashi M, et al.
The translation inhibitor Rocaglamide targets a bimolecular cavity between eIF4A and polypurine RNA.
Molecular Cell
73, 738-748 (2019)
doi: 10.1016/j.molcel.2018.11.026
Kashiwagi K, Takahashi M, Nishimoto M, et al.
Crystal structure of eukaryotic translation initiation factor 2B.
Nature
531, 122-125 (2016)
doi: 10.1038/nature16991
Ito T, Masuda I, Yoshida K, et al.
Structural Basis for methyl-donor-dependent and sequence-specific binding to tRNA substrates by knotted methyltransferase TrmD.
Proceedings of the National Academy of Sciences of the United States of America
112, E4197-E4205 (2015)
doi: 10.1073/pnas.1422981112
Ito T, Yokoyama S.
Two enzymes bound to one transfer RNA assume alternative conformations for consecutive reactions.
Nature
467, 612-616 (2010)
doi: 10.1038/nature09411
Goto-Ito S, Ito T, Kuratani M, et al.
Tertiary structure checkpoint at anticodon loop modification in tRNA functional maturation.
Nature Structural & Molecular Biology
16, 1109-1115 (2009)
doi: 10.1038/nsmb.1653
Members
Takuhiro Ito
Team Leader
Wakana Iwasaki
Senior Research Scientist
Tsutomu Mikawa
Senior Research Scientist
Kazuhiro Kashiwagi
Research Scientist
Sakurako Goto
Research Scientist
Madoka Nishimoto
Technical Staff I
Mari Takahashi
Technical Staff I
Ayako Sakamoto
Technical Staff I
Kayo Nagata
Research Part-time Worker I
