logomark
Research

Research

BDR researchers coming from diverse research fields are working together to achieve higher goals.

Seminars & Symposia

Seminars & Symposia

BDR hosts annual symposium and regular seminars inviting international scientists in life science.

Careers & Study

Careers & Study

BDR embraces people from diverse backgrounds, and strives to create an open and supportive setting for research.

Outreach

Outreach

BDR communicates the appeal and significance of our research to society through the use of various media and activities.

News

News

From research, events, people and everything in between, find out what’s going on at RIKEN BDR.

About Us

About Us

Exploring the scientific foundations of life through interdisciplinary approaches to address society’s problems.

Photo of Team leder, Takuhiro Ito

Team Leader
Takuhiro Ito Ph.D.

Laboratory for Translation Structural Biology

LocationYokohama

E-mailtakuhiro.ito[at]riken.jp

Please replace [at] with @.

Reveals the mechanisms of translation

We study three dimensional structures of the translation factors and/or the ribosome, mainly by X-ray crystallography and cryo-EM. We focus on the structural analysis of the large complexes that function in the eukaryotic translational system, in order to reveal the complicated mechanisms of eukaryotic translation.

Research Theme

  • Structural biology on translational system in eukaryotes
  • Development of the sample preparation methods suitable for the crystallization/cryo-EM analysis of macromolecular complexes

Selected Publications

Kashiwagi K, Shichino Y, Osaki T, et al.
eIF2B-capturing viral protein NSs suppresses the integrated stress response.
Nature Communications 12, 7102 (2021) doi: 10.1038/s41467-021-27337-x

Zyryanova AF, Kashiwagi K, Rato C, et al.
ISRIB blunts the integrated stress response by allosterically antagonising the inhibitory effect of phosphorylated eIF2 on eIF2B.
Molecular Cell 81, 88-103 (2021) doi: 10.1016/j.molcel.2020.10.031

Yokoyama T, Machida K, Iwasaki W, et al.
HCV IRES Captures an Actively Translating 80S Ribosome.
Molecular Cell 74, 1205-1214 (2019) doi: 10.1016/j.molcel.2019.04.022

Kashiwagi K, Yokoyama T, Nishimoto M, et al.
Structural basis for eIF2B inhibition in integrated stress response.
Science 364, 495-499 (2019) doi: 10.1126/science.aaw4104

Iwasaki S, Iwasaki W, Takahashi M, et al.
The translation inhibitor Rocaglamide targets a bimolecular cavity between eIF4A and polypurine RNA.
Molecular Cell 73, 738-748 (2019) doi: 10.1016/j.molcel.2018.11.026

Kashiwagi K, Takahashi M, Nishimoto M, et al.
Crystal structure of eukaryotic translation initiation factor 2B.
Nature 531, 122-125 (2016) doi: 10.1038/nature16991

Ito T, Masuda I, Yoshida K, et al.
Structural Basis for methyl-donor-dependent and sequence-specific binding to tRNA substrates by knotted methyltransferase TrmD.
Proceedings of the National Academy of Sciences of the United States of America 112, E4197-E4205 (2015) doi: 10.1073/pnas.1422981112

Ito T, Yokoyama S.
Two enzymes bound to one transfer RNA assume alternative conformations for consecutive reactions.
Nature 467, 612-616 (2010) doi: 10.1038/nature09411

Goto-Ito S, Ito T, Kuratani M, et al.
Tertiary structure checkpoint at anticodon loop modification in tRNA functional maturation.
Nature Structural & Molecular Biology 16, 1109-1115 (2009) doi: 10.1038/nsmb.1653

Members

Takuhiro Ito

Team Leader

Wakana Iwasaki

Senior Research Scientist

Kazuhiro Kashiwagi

Research Scientist

Sakurako Goto

Research Scientist

Madoka Nishimoto

Technical Scientist

Mari Takahashi

Technical Scientist

Ayako Sakamoto

Technical Scientist

PAGE
TOP