Team Director
Masaya Hagiwara
Ph.D.
Human Biomimetic System RIKEN Hakubi Research Team
LocationKobe / Integrated Innovation Bldg.
E-mailmasaya.hagiwara@riken.jp
The overall objective is the development of in-vitro experimental platform to reconstitute the biological system outside our bodies by controlling cellular micro-environments. Especially lung airway is the one of my major targets.The scientific question is how the airway cells can organize such a sophisticated structure by themselves. The repetitive feedbacks between in-vitro experiments and mathematical model give us a tremendous amount of useful information, and I am challenging to unveil the system underlying self-organization of tissue formation by directing complex tissue structure. Development of Organ or Body-on-a-Chip system is another research target for the animal testing alternative.
Selected Publications
Koh I, Hagiwara M.
Modular tissue-in-a-CUBE platform to model blood-brain barrier (BBB) and brain interaction.
Communications Biology
7(1), 177 (2024)
doi: 10.1038/s42003-024-05857-8
Koh I, Hagiwara M.
Gradient to sectioning CUBE workflow for the generation and imaging of organoids with localized differentiation.
Communications Biology
6(1), 299 (2023)
doi: 10.1038/s42003-023-04694-5
Suthiwanich K, Hagiwara M.
Localization of Multiple Hydrogels with MultiCUBE Platform Spatially Guides 3D Tissue Morphogenesis In Vitro
Advanced Materials Technologies
8(4), 2201660 (2023)
doi: 10.1002/admt.202201660
Takano A, Koh I, Hagiwara M.
3D Culture Platform for Enabling Large-Scale Imaging and Control of Cell Distribution into Complex Shapes by Combining 3D Printing with a Cube Device.
Micromachines
13(2), 156 (2022)
doi: 10.3390/mi13020156
Hagiwara M, Maruyama H, Akiyama M, et al.
Weakening of resistance force by cell-ECM interactions regulate cell migration directionality and pattern formation.
Communications Biology
4(1), 808 (2021)
doi: 10.1038/s42003-021-02350-4
Hagiwara M, Koh I.
Engineering approaches to control and design the in vitro environment towards the reconstruction of organs.
Development, Growth & Differentiation
62(3), 158-166 (2020)
doi: 10.1111/dgd.12647
Hagiwara M, Nobata R, Kawahara T.
Initial 3D Cell Cluster Control in a Hybrid Gel Cube Device for Repeatable Pattern Formations.
Journal of Visualized Experiments
(2019)
doi: 10.3791/59214
Hagiwara M, Nakase I.
Epidermal growth factor induced macropinocytosis directs branch formation of lung epithelial cells.
Biochemical and Biophysical Research Communications
507(1-4), 297-303 (2018)
doi: 10.1016/j.bbrc.2018.11.028
Hagiwara M, Nobata R, Kawahara T.
High repeatability from 3D experimental platform for quantitative analysis of cellular branch pattern formations.
Integrative Biology
10(5), 306-312 (2018)
doi: 10.1039/c8ib00032h
Hagiwara M, Nobata R, Kawahara T.
Large Scale Imaging by Fine Spatial Alignment of Multi-Scanning Data with Gel Cube Device
Applied Sciences
8(2), 235 (2018)
doi: 10.3390/app8020235
Hagiwara M, Maruta N, Marumoto M.
In Vitro Experimental Model for the Long-Term Analysis of Cellular Dynamics During Bronchial Tree Development from Lung Epithelial Cells.
Tissue Engineering. Part C, Methods
23(6), 323-332 (2017)
doi: 10.1089/ten.TEC.2017.0126
Hagiwara M.
An in vitro-in silico interface platform for spatiotemporal analysis of pattern formation in collective epithelial cells.
Integrative Biology
8(8), 861-868 (2016)
doi: 10.1039/c6ib00073h
Hagiwara M, Kawahara T, Nobata R.
Tissue in Cube: In Vitro 3D Culturing Platform with Hybrid Gel Cubes for Multidirectional Observations.
Advanced Healthcare Materials
5(13), 1566-1571 (2016)
doi: 10.1002/adhm.201600167
Members
Masaya Hagiwara
RIKEN Hakubi Team Leader
Yen Xuan Ngo
Postdoctoral Researcher
Marlar Saw
Technical Staff I
Satoko Kinoshita
Assistant
