Team Leader
Toshio Yanagida
Ph.D.
Laboratory for Single Cell Mass Spectrometry
[Closed Mar. 2021]
Cells, the building blocks of life, show various responses to stimuli. Clarification of these cellular dynamics by comprehensive and quantitative evaluation of the changes over time can help us to understand various aspects of life, such as cellular responses to environmental changes, molecular mechanisms of development, and mechanisms of carcinogenesis of cells etc. The Laboratory for Single Cell Mass Spectrometry aims to develop technologies to comprehensively reveal intracellular metabolites and proteins at a single cell level by using a single cell mass spectrometry technique with a nanospray chip and a capillary electrophoresis mass spectrometry technique. We also aim to further develop the established technologies for single cell drug discovery and novel diagnostic techniques.
Research Theme
- Development of single cell mass spectrometry
- Development of high sensitivity capillary electrophoresis mass spectrometry
- Development of single cell drug discovery/single cell diagnosis
Selected Publications
Kawai T, Mihara Y, Morita M, et al.
Quantitation of Cell Membrane Permeability of Cyclic Peptides by Single-Cell Cytoplasm Mass Spectrometry.
Analytical Chemistry (2021) doi: 10.1021/acs.analchem.0c03901
Kawai T, Ota N, Okada K, et al.
Ultrasensitive single cell metabolomics by capillary electrophoresis–mass spectrometry with a thin-walled tapered emitter and large-volume dual sample preconcentration.
Analytical Chemistry 91, 10564–10572 (2019) doi: 10.1021/acs.analchem.9b01578
Ali A, Abouleila Y, Shimizu Y, et al.
Single-cell screening of tamoxifen abundance and effect using mass spectrometry and Raman-spectroscopy.
Analytical chemistry 91(4). 2710-2718 (2019) doi: 10.1021/acs.analchem.8b04393
Kawai T, Ota N, Imasato A, et al.
Profiling of N-linked glycans from 100 cells by capillary electrophoresis with large-volume dual preconcentration by isotachophoresis and stacking
Journal of Chromatography A 1563. 138-144 (2018) doi: 10.1016/j.chroma.2018.06.034
Narumi R, Masuda K, Tomonagaa T, et al.
Cell-free synthesis of stable isotope-labeled internal standards for targeted quantitative proteomics.
Synthetic and Systems Biotechnology 3. 97-104 doi:10.1016/j.synbio.2018.02.004
Masuda K, Abouleila Y, Ali A, et al.
Live Single-Cell Mass Spectrometry for Plant Metabolomics.
Methods in Molecular Biology 1778. 269-282 (2018) doi:10.1007/978-1-4939-7819-9_19
Emara S, Amer S, Ali A, et al.
Single-Cell Metabolomics.
Advances in Experimental Medicine and Biology 965. 323-343 (2017) doi:10.1007/978-3-319-47656-8_13
Ali A, Abouleila Y, Amer S, et al.
Quantitative Live Single-cell Mass Spectrometry with Spatial Evaluation by Three-Dimensional Holographic and Tomographic Laser Microscopy.
Analytical Sciences 32(2). 125-127 (2016) doi:10.2116/analsci.32.125
Yamamoto K, Takahashi K, Mizuno H, et al.
Cell-specific localization of alkaloids in Catharanthus roseus stem tissue measured with Imaging MS and Single-cell MS.
Proceedings of the National Academy of Sciences of the United States of America 113(14). 3891-3896 (2016) doi:10.1073/pnas.1521959113
Hiyama E, Ali A, Amer S, et al.
Direct Lipido-Metabolomics of Single Floating Cells for Analysis of Circulating Tumor Cells by Live Single-cell Mass Spectrometry.
Analytical Sciences 31(12). 1215-1217 (2015) doi:10.2116/analsci.31.1215
Esaki T, Masujima T.
Fluorescence Probing Live Single-cell Mass Spectrometry for Direct Analysis of Organelle Metabolism.
Analytical Sciences 31(12). 1211-1213. (2015) doi:10.2116/analsci.31.1211
Fujii T, Matsuda S, Tejedor ML, et al.
Direct metabolomics for plant cells by live single-cell mass spectrometry.
Nature Protocols 10(9). 1445-1456 (2015) doi:10.1038/nprot.2015.084
Emara S, Masujima T, Zarad W et al.
An Eco-Friendly Direct Injection HPLC Method for Methyldopa Determination in Serum by Mixed-Mode Chromatography Using a Single Protein-Coated Column.
Journal of Chromatographic Science 53(8). 1353-1360 (2015) doi:10.1093/chromsci/bmv024
Fujita H, Esaki T, Masujima T, et al.
Comprehensive chemical secretory measurement of single cells trapped in a micro-droplet array with mass spectrometry.
Rsc Advances 5(22). 16968-16971 (2015) doi:10.1039/c4ra12021c
