Laboratory for Advanced Biomolecular Engineering | RIKEN BDR

Laboratory for Advanced Biomolecular Engineering

Unit Leader

Shunsuke TagamiPh.D.

  • Location:Yokohama
  • E-mail:shunsuke.tagami[at]riken.jpPlease replace [at] with @.

We are developing biomacromolecules that control enzymes and cells.

Research Summary

We are engineering functional biomacromolecules applicable to medical and basic researches, by integrating technologies in synthetic and structural biology including peptide/RNA in vitro evolution, X-ray crystallography, etc. Utilizing the same technologies, we are also trying to reconstruct the evolutional pathway of primordial proteins.

Research Theme

  • We are engineering peptides that control cells and enzymes for use in medical and basic science.
  • We are trying to experimentally reconstruct evolutional pathway from primordial peptides to elaborate proteins.

Main Publications List

  • Tagami S, Attwater J, Holliger P.
    Simple peptides derived from the ribosomal core potentiate RNA polymerase ribozyme function.
    Nature Chemistry 9. 325-332 (2017) doi: 10.1038/nchem.2739.
  • Tagami S, Sekine S, Minakhin L, et al.
    Structural basis for promoter specificity switching of RNA polymerase by a phage factor.
    Genes & Development 28(5). 521-531 (2014) doi: 10.1101/gad.233916.113
  • Attwater J, Tagami S, Kimoto M, et al.
    Chemical fidelity of an RNA polymerase ribozyme.
    Chemical Science 4. 2804–2814 (2013) doi: 10.1039/C3SC50574J
  • Sekine S, Tagami S, Yokoyama S
    Structural basis of transcription by bacterial and eukaryotic RNA polymerases.
    Current Opinion in Structural Biology 22(1). 110-118 (2012) doi: 10.1016/j.sbi.2011.11.006
  • Tagami S, Sekine S, Yokoyama S.
    A novel conformation of RNA polymerase sheds light on the mechanism of transcription.
    Transcription 2(4). 162-167 (2011) doi: 10.4161/trns.2.4.16148
  • Tagami S, Sekine S, Kumarevel T, et al.
    Crystal structure of bacterial RNA polymerase bound with a transcription inhibitor protein.
    Nature 468(7326). 978-982 (2010) doi: 10.1038/nature09573
  • Tagami S, Sekine S, Kumarevel T, et al.
    Crystallization and preliminary X-ray crystallographic analysis of Thermus thermophilus transcription elongation complex bound to Gfh1.
    Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS 66(Pt1). 63-68 (2010) doi: 10.1038/nature09573

Member

Shunsuke TagamiUnit Leader shunsuke.tagami[at]riken.jp CV
Sota YagiResearch Scientist sota.yagi[at]riken.jp CV
Peiying LiResearch Associate peiying.li[at]riken.jp
Tami KosugeAssistant tami.kosuge[at]riken.jp

*:concurrent / Please replace [at] with @.