Team Leader
Ichio Shimada
Ph.D.
Laboratory for Dynamic Structure of Biomolecules
LocationYokohama
E-mailichio.shimada[at]riken.jp
Please replace [at] with @.
Membrane proteins play fundamental roles in many biological processes, and are recognized as principal target proteins for drug development. RNAs have become recognized asa novel remedy to solve the depletion of targets in drug development.Over the past decade, our structural understanding of biomolecules such as the membrane proteins and RNAshas dramatically progressed, owing to the growing numbers of their atomic resolution crystal and cryo-EM structures. However, these structures basically represent static snapshots and observed conformations may not be the same as those in in-situ environment. In this research team, by using NMR, which provides us information about dynamical structures of biomolecules in solution, we will investigate the relationships between the dynamical structures and the functions for biologically important biomolecules.
Research Theme
- Elucidation of functional mechanism of biomolecules from dynamic structures
- Elucidation of signal transduction mechanism of GPCRs from dynamic structures
- Development of methods for dynamic structures of membrane proteins in lipid bilayers
- Development of NMR methods applicable to high-molecular-weight biomolecules
Selected Publications
Imai S, Suzuki H, Fujiyoshi Y, et al.
Dynamically regulated two-site interaction of viral RNA to capture host translation initiation factor.
Nature Communications
14, 4977 Fri Sep 01 00:00:00 JST 2023
doi: 10.1038/s41467-023-40582-6
Shiraishi Y, Shimada I.
NMR Characterization of the Papain-like Protease from SARS-CoV-2 Identifies the Conformational Heterogeneity in Its Inhibitor-Binding Site.
Journal of the American Chemical Society
145, 16669-16677 Fri Sep 01 00:00:00 JST 2023
doi: 10.1021/jacs.3c04115
Kaneko S, Imai S, Asao N, et al.
Activation mechanism of the μ-opioid receptor by an allosteric modulator.
Proceedings of the National Academy of Sciences
119(16), e2121918119 Mon Apr 11 00:00:00 JST 2022
doi: 10.1073/pnas.2121918119
Shiraishi Y, Kofuku Y, Ueda T, et al.
Biphasic activation of β-arrestin 1 upon interaction with a GPCR revealed by methyl-TROSY NMR
Nature Communications
12, 7158 Thu Dec 09 00:00:00 JST 2021
doi: 10.1038/s41467-021-27482-3
Iwahashi Y, Toyama Y, Imai S, et al.
Conformational equilibrium shift underlies altered K+ channel gating as revealed by NMR.
Nature Communications
11, 5168 Tue Dec 01 00:00:00 JST 2020
doi: 10.1038/s41467-020-19005-3
Mizukoshi Y, Takeuchi K, Tokunaga Y, et al.
Targeting the cryptic sites: NMR-based strategy to improve protein druggability by controlling the conformational equilibrium.
Science Advances
6(40), eabd0480 Sun Nov 01 00:00:00 JST 2020
doi: 10.1126/sciadv.abd0480
Mizumura T, Kondo K, Kurita M, et al.
Activation of adenosine A2A receptor by lipids from docosahexaenoic acid revealed by NMR.
Science Advances
6(12), eaay8544 Tue Sep 01 00:00:00 JST 2020
doi: 10.1126/sciadv.aay8544
Imai S, Yokomizo T, Kofuku Y, et al.
Structural equilibrium underlying ligand -dependent activation of β2 -adreno receptor.
Nature Chemical Biology
16, 430-439 Sat Aug 01 00:00:00 JST 2020
doi: 10.1038/s41589-019-0457-5
Kano H, Toyama Y, Imai S, et al.
Structural mechanism underlying G protein family-specific regulation of G protein-gated inwardly rectifying potassium channel.
Nature Communications
10(1), 2008 Sun Dec 01 00:00:00 JST 2019
doi: 10.1038/s41467-019-10038-x
Shimada I, Ueda T, Kofuku Y, et al.
GPCR drug discovery: integrating solution NMR data with crystal and cryo-EM structures.
Nature Reviews Drug Discovery
18(1), 59-82 Fri Nov 01 00:00:00 JST 2019
doi: 10.1038/nrd.2018.180
Members
Ichio Shimada
Team Leader
Shunsuke Imai
Senior Scientist
Fumiaki Hayashi
Senior Scientist
Yutaro Shiraishi
Research Scientist
Yuki Toyama
Research Scientist
Satoko Tamura
Technical Staff I
Shun Kaneko
Student Trainee
