Characteristics of bacterial regulatory small RNAs
Jan. 30, 2020 13:30 - 15:00
C210-212, Yokohama Central Research Building
Faculty of Pharmaceutical Sciences, Suzuka University of Medical Science
Regulation at post-transcriptional steps allows new pathways to cross-regulate genes independently of the transcriptional signals for those genes. Regulatory small RNAs (sRNAs), which act as a post-transcriptional regulator, are transcribed under specific physiological/stress conditions. The sRNAs interact with mRNAs by base-pairing, resulting in changes in the translation and stability of the target mRNAs. In gram-negative bacteria, the base-pairing is facilitated in general by the RNA chaperon Hfq.
E. coli sRNAs are around 100 nucleotides in length, and contain at least two functional regions, an mRNA base-pairing region and an Hfq-binding region essentially overlapping with a Rho-independent terminator sequence. The base-pairing region is partially complementary to the translation initiation region of target mRNAs. The sRNA-mRNA base pairing leads mostly to inhibition of translation of target mRNAs. The Hfq-binding region of the sRNAs consists of a polyU tail and a U-rich sequence immediately upstream of stem loop structure. The polyU tail must be longer than seven for the Hfq-binding. Both the 3'-extended transcripts resulting from read-through and the 3'-shortened transcripts resulting from premature termination no longer function as sRNAs. Discussion will focus on new insights into roles of stem loop structure in Rho-independent terminators of sRNAs.