1. tRNA Methylation Is a Global Determinant of Bacterial Multi-Drug Resistance 2. Insights into Genome Recoding from the Mechanism of a Classic +1-Frameshift-Suppressor tRNA
Nov. 14, 2019 15:30 - 17:00
1. Ya-Ming Hou 2. Howard Gamper
Department of Biochemistry and Molecular Biology, Thomas Jefferson University
Gram-negative bacteria are intrinsically resistant to drugs. Here, we show that m1G37-tRNA methylation determines the synthesis of a multitude of membrane proteins via its control of translation at proline codons near the start of open-reading frames. Our results highlight the potential of tRNA methylation in codon-specific translation to control the development of multi-drug resistance in Gram-negative bacteria.
While quadruplet codons are attractive for genome recoding, their translation mechanism is unknown. Using a classic +1-frameshift-suppressor tRNA SufB2 as a model, we elucidated the mechanism by which quadruplet codons are translated by +1-frameshifting. Our results highlight the potential of ribosomal head-domain-mediated translocation as a regulator of +1-frameshifting and suggest that successful engineering of head swiveling can improve the efficiency of genome recoding.