Team Leader
Tomonobu Watanabe
Ph.D.
Laboratory for Comprehensive Bioimaging
Location :Kobe / Developmental Biology Buildings, Hiroshima
E-mailtomowatanabe[at]riken.jp
Please replace [at] with @.
Light has been an essential modality in medical and life science for 400 years because of the availability of living specimens. When light passes through the biomaterial or when light emanates from the biomaterial, the light is modified/modulated by information inside the biomaterial. Our developmental aim is extract the biological information from the fluorescence or scattering light. We are also trying to apply the developed measurement technology to various fields such as medicine and agriculture. For example, Raman scattering light contains all the molecular information constituting a substance, and the Raman scattered light spectrum of the cell is very complicated. We exploit this complexity to make Raman scattered light spectrum into a cellular fingerprint that identifies the type and state of cells. In addition, this "cell fingerprinting technology" is developed as an evaluation method applicable without touching or damaging cells or tissues. We are conducting research and development on as many innovation seeds as possible by simultaneously developing both basic measurement principles and practical application techniques. We believe that these technologies support many biological research fields and contribute to industries and revitalize the community.
Research Theme
- Stem cell research by utilizing single molecule measurement for transcription factors.
- Development and application of cellular fingerprinting based on Raman spectroscopy
- Development and application of protein structural analysis based on second harmonic generation microscopy
- Development of whole tissue imaging
- The others
Selected Publications
Kawai T, Mihara Y, Morita M, et al.
Quantitation of Cell Membrane Permeability of Cyclic Peptides by Single-Cell Cytoplasm Mass Spectrometry.
Analytical chemistry
93(7), 3370-3377 Tue Feb 23 00:00:00 JST 2021
doi: 10.1021/acs.analchem.0c03901
Germond A, Panina Y, Shiga M, et al.
Following Embryonic Stem Cells, Their Differentiated Progeny, and Cell-State Changes During iPS Reprogramming by Raman Spectroscopy.
Analytical Chemistry
92, 14915-14923 Tue Dec 01 00:00:00 JST 2020
doi: 10.1021/acs.analchem.0c01800
Kakizuka T, Takai A, Yoshizawa K, et al.
An improved fluorescent protein-based expression reporter system that utilizes bioluminescence resonance energy transfer and peptide-assisted complementation
Analytical chemistry
56, 3625-3628 Wed Feb 19 00:00:00 JST 2020
doi: 10.1039/C9CC08664A
David BG, Fujita H, Yasuda K, et al.
Linking substrate and nucleus via actin cytoskeleton in pluripotency maintenance of mouse embryonicstem cells.
Nat Protoc.
41, 101614 Sun Dec 01 00:00:00 JST 2019
doi: 10.1016/j.scr.2019.101614
Matsumoto K, Mitani TT, Horiguchi SA, et al.
Advanced CUBIC tissue clearing for whole-organ cell profiling
Nat Protoc.
14, 3506-3537 Wed Nov 20 00:00:00 JST 2019
doi: 10.1038/s41596-019-0240-9.
Kaneshiro J, Okada Y, Shima T, et al.
Second harmonic generation polarization microscopy as a tool for protein structure analysis.
Biophys Physicobiol.
16, 147-157 Fri Sep 20 00:00:00 JST 2019
doi: 10.2142/biophysico.16.0_147
Germond A, Ichimura T, Horinouchi T, et al.
Raman spectral signature reflects transcriptomic features of antibiotic resistance.
Communications Biology
1, 85 Sat Dec 01 00:00:00 JST 2018
doi: 10.1038/s42003-018-0093-8
Okamoto K, Germond A, Fujita H, et al.
Single cell analysis reveals a biophysical aspect of collective cell-state transition in embryonic stem cell differentiation.
Sci Rep.
8, 11965 Fri Aug 10 00:00:00 JST 2018
doi: 10.1038/s41598-018-30461-2
Morikawa TJ, Fujita H, Kitamura A, et al.
Dependence of fluorescent protein brightness on protein concentration in solution and enhancement of it.
Scientific Reports
6, 22342 Sat Oct 01 00:00:00 JST 2016
doi: 10.1038/srep22342
Ichimura T, Chiu L, Fujita K, et al.
Visualizing the appearance and disappearance of the attractor of differentiation using Raman spectral imaging.
Scientific Reports
5, 11358 Tue Dec 01 00:00:00 JST 2015
doi: 10.1038/srep11358
Members
Tomonobu Watanabe
Team Leader
Yasuhiro Maeda
Technical Scientist
Go Shioi
Technical Scientist
Junya Ichinose
Research Scientist
Michiko Inoue
Technical Staff I
Yuki Tano
Assistant