Team Leader
Yu-Chiun Wang Ph.D.

The central question in developmental biology is how cells, tissues and organs acquire their specific functions and shapes. A large body of work over the past several decades has yielded a broad understanding of how functional specialization is achieved through differential gene expression. In contrast, far less is known about how cell shapes and tissue structures are controlled and remodeled. Although a general theme has emerged whereby cytoskeletal elements control the cell shapes, while alteration of individual cell shapes collectively organizes the tissue architecture, the underlying molecular and mechanical mechanisms remain poorly understood. My lab aims at identifying novel mechanisms that orchestrate the formation of three-dimensional epithelial structures. Our long-term goal is to comprehensively understand the mechanistic principles of tissue morphogenesis in order to conceptualize the origin of morphological diversity both within an organism and among evolutionary lineages.

We are currently focusing on how modifications of epithelial cell polarity control cell shapes using gastrulating Drosophila embryos as the model system. Our recent work identified a novel microtubule based mechanical mechanism that is crucial for maintaining homogeneities of cell sizes and shapes prior to morphogenesis, and yet becomes coupled to the changing cell polarity, thus repurposed for cell shortening that induces folding of the epithelial tissue. The polarity-based, microtubule-dependent mechanism contrasts with the canonical myosin-dependent apical constriction. Our ongoing work promises to delineate a novel mechanical force balance/imbalance process that underlies non-myosin based epithelial folding.

We employ an integrated approach that combines genetic manipulation, quantitative live imaging and computational mechanical modeling. We are also in the process of developing optogenetic and cell-type specific RNA-seq methodologies that could be used to manipulate and identify factors and parameters that control cell shape change and tissue deformation. Furthermore, our lab is engaged in international, multidisciplinary collaborations with scientists that specialize in evolutionary biology, computational mechanics, and theoretical physics to seek to better understand epithelial morphogenesis from a variety of different angles.

Research Theme

• How does modification of cell polarity cause cell shape change and bending of an epithelial tissue?
• How does physical coupling between adherens junctions and actin control the extent of invagination?
• Developing strategies for quantitative 4D imaging and visualization of forces during morphogenesis
• The evolutionary possibilities of novel and temporary morphogenetic structures