Team Leader
Masahiro Ueda
Ph.D.
Laboratory for Cell Signaling Dynamics
LocationOsaka / Osaka University BioSystems Building
E-mailmasahiroueda[at]riken.jp
Please replace [at] with @.
Our group studies how signal transduction function of living cells arises spontaneously from the dynamics of intracellular reaction networks (design principles), and the mechanisms that allow these systems to process signals under the strong influence of thermal and stochastic noise (computation principles). As a typical example of stochastic biomolecular computation in living cells, we specifically focus on the gradient sensing and directional cell migration seen in the cellular slime mold Dictyostelium discoideum. In this research project, by combining advanced imaging techniques including single-molecule detection with theoretical modeling, we aim to better understand these two principles while at the same time develop the techniques necessary to do so.
Research Theme
- Spontaneous signal generation in living motile cells
- Stochastic signal transduction and processing
- Automated single-molecule imaging analysis in living cells
- Organized randomness in hierarchical structure of biological systems
Selected Publications
Miyagawa T, Koteishi H, Kamimura Y, et al.
Structural basis of Gip1 for cytosolic sequestration of G protein in wide-range chemotaxis.
Nature Communications
9, 4635 Sat Dec 01 00:00:00 JST 2018
doi: 10.1038/s41467-018-07035-x
Matsuoka S, Ueda M.
Mutual inhibition between PTEN and PIP3 generates bistability for polarity in motile cells.
Nature communications
9(1), 4481 Fri Oct 26 00:00:00 JST 2018
doi: 10.1038/s41467-018-06856-0
Yasui M, Hiroshima M, Kozuka J, et al.
Automated single-molecule imaging in living cells.
Nature Communications
9, 3061 Mon Oct 01 00:00:00 JST 2018
doi: 10.1038/s41467-018-05524-7
Yanagawa M, Hiroshima M, Togashi Y, et al.
Single-molecule diffusion-based estimation of ligand effects on G protein-coupled receptors.
Science signaling
11(548), eaao1917 Tue Sep 18 00:00:00 JST 2018
doi: 10.1126/scisignal.aao1917
Kamimura Y, Miyanaga Y, Ueda M.
Heterotrimeric G-protein shuttling via Gip1 extends the dynamic range of eukaryotic chemotaxis.
Proceedings of the National Academy of Sciences of the United States of America
113(16), 4356-4361 Thu Dec 01 00:00:00 JST 2016
doi: 10.1073/pnas.1516767113
Komatsuzaki A, Ohyanagi T, Tsukasaki Y, et al.
Compact Halo-ligand-conjugated quantum dots for multicolored single-molecule imaging of overcrowding GPCR proteins on cell membranes.
Small
11(12), 1396-1401 Tue Dec 01 00:00:00 JST 2015
doi: 10.1002/smll.201402508
Yasui M, Matsuoka S, Ueda M.
PTEN Hopping on the Cell Membrane Is Regulated via a Positively-Charged C2 Domain.
Plos Computational Biology
10(9), e1003817 Mon Dec 01 00:00:00 JST 2014
doi: 10.1371/journal.pcbi.1003817
Cai H, Katoh-Kurasawa M, Muramoto T, et al.
Nucleocytoplasmic Shuttling of a GATA Transcription Factor Functions as a Development Timer.
Science
343(6177), 1329-+ Sat Nov 01 00:00:00 JST 2014
doi: 10.1126/science.1249531
Nishikawa M, Horning M, Ueda M, Shibata T.
Excitable Signal Transduction Induces Both Spontaneous and Directional Cell Asymmetries in the Phosphatidylinositol Lipid Signaling System for Eukaryotic Chemotaxis.
Biophysical Journal
106(3), 723-734 Wed Oct 01 00:00:00 JST 2014
doi: 10.1016/j.bpj.2013.12.023
Okamoto M, Namba T, Shinoda T, et al.
TAG-1-assisted progenitor elongation streamlines nuclear migration to optimize subapical crowding.
Nature Neuroscience
16(11), 1556-1566 Sun Dec 01 00:00:00 JST 2013
doi: 10.1038/nn.3525
Matsuoka S, Shibata T, Ueda M.
Asymmetric PTEN Distribution Regulated by Spatial Heterogeneity in Membrane-Binding State Transitions.
Plos Computational Biology
9(1), e1002862 Fri Nov 01 00:00:00 JST 2013
doi: 10.1371/journal.pcbi.1002862
Nishimura SI, Ueda M, Sasai M.
Non-Brownian dynamics and strategy of amoeboid cell locomotion.
Physical Review E
85(4), 041909 Sat Dec 01 00:00:00 JST 2012
doi: 10.1103/PhysRevE.85.041909
Tsujioka M, Yumura S, Inouye K, et al.
Talin couples the actomyosin cortex to the plasma membrane during rear retraction and cytokinesis.
Proceedings of the National Academy of Sciences of the United States of America
109(32), 12992-12997 Thu Nov 01 00:00:00 JST 2012
doi: 10.1073/pnas.1208296109
Arai Y, Shibata T, Matsuoka S, et al.
Self-organization of the phosphatidylinositol lipids signaling system for random cell migration.
Proceedings of the National Academy of Sciences of the United States of America
107(27), 12399-12404 Wed Dec 01 00:00:00 JST 2010
doi: 10.1073/pnas.0908278107
