Team Leader
Mikako Shirouzu
Ph.D.
Laboratory for Protein Functional and Structural Biology
LocationYokohama
E-mailmikako.shirouzu[at]riken.jp
Please replace [at] with @.
The high-resolution structural information of proteins related to diseases shall increasingly become important for drug development leading to future individualized medicine. We plan to establish a structural analysis technology platform to contribute to "Life innovation" such as drug discovery as well as development of methods for the sample preparation of challenging proteins including membrane proteins/biomolecular complexes and for the structural analysis by cryo-electron microscopy (cryo-EM). The 3D-structural information will be used for in-silico screening/design of chemical compounds and for dynamic structural analysis toward simulation research of cell function.
Research Theme
- Analysis of developmental process using theoretical cell models
- Study of evolutionary dynamics of microorganisms by comprehensive phenotypic/genetic analysis
- Development of algorithm for high-resolution comprehensive phenotypic/genetic analysis
Selected Publications
Akiyama N, Ishiguro K, Yokoyama T, et al.
Structural insights into the decoding capability of isoleucine tRNAs with lysidine and agmatidine.
Nature Structural & Molecular Biology
Wed Mar 27 00:00:00 JST 2024
doi: 10.1038/s41594-024-01238-1
Suzuki M, Uchibori K, Oh-Hara T, et al.
A macrocyclic kinase inhibitor overcomes triple resistant mutations in EGFR-positive lung cancer.
Npj Precision Oncology
8(1), 46 Fri Feb 23 00:00:00 JST 2024
doi: 10.1038/s41698-024-00542-9
Tanaka M, Yokoyama T, Saito H, et al.
Boric acid intercepts 80S ribosome migration from AUG-stop by stabilizing eRF1.
Nature Chemical Biology
Wed Jan 24 00:00:00 JST 2024
doi: 10.1038/s41589-023-01513-0
Furutani Y, Hirano Y, Toguchi M, et al.
A small molecule iCDM-34 identified by in silico screening suppresses HBV DNA through activation of aryl hydrocarbon receptor.
Cell Death Discovery
9(1), 467 Fri Dec 22 00:00:00 JST 2023
doi: 10.1038/s41420-023-01755-w
Zhao X, Ma D, Ishiguro K, et al.
Glycosylated queuosines in tRNAs optimize translational rate and post-embryonic growth.
Cell
186(25), 5517-5535.e24 Thu Dec 07 00:00:00 JST 2023
doi: 10.1016/j.cell.2023.10.026
Kikuchi M, Morita S, Wakamori M, et al.
Epigenetic mechanisms to propagate histone acetylation by p300/CBP.
Nature Communications
14(1), 4103 Mon Jul 17 00:00:00 JST 2023
doi: 10.1038/s41467-023-39735-4
Yamagata A, Murata Y, Namba K, et al.
Uptake mechanism of iron-phytosiderophore from the soil based on the structure of yellow stripe transporter.
Nature Communications
13, 7180 Wed Nov 23 00:00:00 JST 2022
doi: 10.1038/s41467-022-34930-1
Ehara H, Kujirai T, Shirouzu M, et al.
Structural basis of nucleosome disassembly and reassembly by RNAPII elongation complex with FACT.
Science
377(6611), eabp9466 Fri Sep 09 00:00:00 JST 2022
doi: 10.1126/science.abp9466
Nakamura H, Hisano T, Rahman MM, et al.
Structural basis for heme detoxification by an ATP-binding cassette-type efflux pump in gram-positive pathogenic bacteria.
Proceedings of the National Academy of Sciences of the United States of America
119(27), e2123385119 Tue Jul 05 00:00:00 JST 2022
doi: 10.1073/pnas.2123385119
Park JH, Iwamoto M, Yun JH, et al.
Structural insights into the HBV receptor and bile acid transporter NTCP.
Nature
606(7916), 1027-1031 Wed Jun 01 00:00:00 JST 2022
doi: 10.1038/s41586-022-04857-0
Hosaka T, Nomura T, Kubo M, et al.
Conformational alterations in unidirectional ion transport of a light-driven chloride pump revealed using X-ray free electron lasers.
Proceedings of the National Academy of Sciences of the United States of America
119(9), e2117433119 Tue Mar 01 00:00:00 JST 2022
doi: 10.1073/pnas.2117433119
Nakagawa Y, Shen HC, Komi Y, et al.
Amyloid conformation-dependent disaggregation in a reconstituted yeast prion system.
Nature Chemical Biology
18(3), 321-331 Tue Mar 01 00:00:00 JST 2022
doi: 10.1038/s41589-021-00951-y
Kasahara K, Re S, Nawrocki G, et al.
Reduced Efficacy of a Src Kinase Inhibitor in Crowded Protein Solution.
Nature Communications
12, 4099 Wed Dec 01 00:00:00 JST 2021
doi: 10.1038/s41467-021-24349-5
Kukimoto-Niino M, Katsura K, Kaushik R, et al.
Cryo-EM structure of the human ELMO1-DOCK5-Rac1 complex.
Science Advances
7(30), eabg3147 Thu Jul 01 00:00:00 JST 2021
doi: 10.1126/sciadv.abg3147
News
Mar. 16, 2023 Research
A nifty trick to help plants thrive in iron-poor soils
Jun. 9, 2022 Research
Imaging how a light-driven chloride pump works
Nov. 1, 2021 Research
Mapping the structural features that regulate a signaling protein’s specificity
Aug. 9, 2019 Research
How phosphorylation of eIF2 reduces protein synthesis
Dec. 7, 2018 Research
Cryo-electron microscopy shows how stabilizing proteins bind to microtubules