Laboratory for Molecular and Cellular Dynamics

Unit Leader

Yuko Mimori-KiyosuePh.D.

  • Location:Kobe / Developmental Biology Buildings
  • E-mail:yuko.kiyosue[at]riken.jpPlease replace [at] with @.
  • Lab Website

Biomolecular and cellular functions highlighted by leading-edge light microscopy technologies

Research Area

It is the working capacity of individual cells in our body that ensures we remain healthy or causes disease. The activities of cells are expressed through signal transduction, infrastructure construction and their dynamic regulation in a spatiotemporal manner. We are exploring the molecular and cellular mechanisms underlying normal development and diseases by employing a 3D high-resolution live-imaging technology, “lattice light-sheet microscopy”, which provides cellular information with unprecedented accuracy and precision [1, 2]. Currently, progress to establish a new style of cell biology using a large quantity of multidimensional information from lattice light-sheet microscopy is ongoing.

A time-lapse movie of microtubules (red) and EB1-GFP (green).

Research Theme

  • Functional study of intracellular machinery driving life
  • Study of molecular mechanisms to maintain cellular integrity
  • Development of a method for cell function editing by 3D local photostimulation with lattice light-sheet microscope
  • Development of a quantitative cell phenotyping method using lattice light-sheet microscopy

Main Publications List

  • Yamashita N, Morita M, Legant WR, et al.
    Three-dimensional tracking of plus-tips by lattice light-sheet microscopy permits the quantification of microtubule growth trajectories within the mitotic apparatus.
    Journal of Biomedical Optics 20(10). 101206 (2015) doi: 10.1117/1.JBO.20.10.101206
  • Chen BC, Legant WR, Wang K, et al.
    Lattice light-sheet microscopy: imaging molecules to embryos at high spatiotemporal resolution.
    Science 346(6208). 1257998 (2014) doi: 10.1126/science.1257998
  • Shimozawa T, Yamagata K, Kondo T, et al.
    Improving spinning disk confocal microscopy by preventing pinhole cross-talk for intravital imaging.
    Proceedings of the National Academy of Sciences of the United States of America 110(9), 3399-3404 (2013). doi: 10.1073/pnas.1216696110
  • Nakamura S, Grigoriev I, Nogi T,, et al.
    Dissecting the nanoscale distributions and functions of microtubule-end-binding proteins EB1 and ch-TOG in interphase HeLa cells.
    PLOS ONE 7(12). e51442 (2012) doi: 10.1371/journal.pone.0051442
  • Mimori-Kiyosue Y.
    Shaping microtubules into diverse patterns: molecular connections for setting up both ends.
    Cytoskeleton (Hoboken) 68(11). 603-618 (2011) doi: 10.1002/cm.20540
  • Hotta A, Kawakatsu T, Nakatani T, et al.
    Laminin-based cell adhesion anchors microtubule plus ends to the epithelial cell basal cortex through LL5α/β.
    The Journal of Cell Biology 189(5). 901-917 (2010) doi: 10.1083/jcb.200910095
  • Mimori-Kiyosue Y, Grigoriev I, Lansbergen G, et al.
    CLASP1 and CLASP2 bind to EB1 and regulate microtubule plus-end dynamics at the cell cortex.
    The Journal of Cell Biology 168(1). 141-153 (2005) doi: 10.1083/jcb.200405094
  • Mimori-Kiyosue Y, Tsukita S.
    Search-and-capture of microtubules through plus-end-binding proteins (+TIPs).
    The Journal of Biochemistry 134(3). 321-326 (2003) doi: 10.1093/jb/mvg148
  • Mimori-Kiyosue Y, Shiina N, Tsukita S.
    The dynamic behavior of the APC-binding protein EB1 on the distal ends of microtubules.
    Current Biology 10(14). 865-868 (2000) doi: 10.1016/S0960-9822(00)00600-X
  • Mimori-Kiyosue Y, Shiina N, Tsukita S.
    Adenomatous polyposis coli (APC) protein moves along microtubules and concentrates at their growing ends in epithelial cells.
    The Journal of Cell Biology 148(3). 505-518 (2000) doi: 10.1083/jcb.148.3.505

All Publications

Member

Yuko KiyosueUnit Leader yuko.kiyosue[at]riken.jp
Taisaku NogiTechnical Staff I
Yuki TanoAssistant

*:concurrent / Please replace [at] with @.