Team Leader
Hiroshi Kiyonari
Ph.D.
Laboratory for Animal Resources and Genetic Engineering
LocationKobe / MI R&D Center Building
E-mailhiroshi.kiyonari[at]riken.jp
Please replace [at] with @.
- Harnessing Experimental Animals -
The major mission of the Animal Resource Development Unit is to integrate advanced reproductive and developmental biology technologies and environment for animal research experiments through innovative research in the development of novel experimental animal models and related technical support activities. We utilize our expertise, not only to manage the state-of-the-art SPF rodent facility at RIKEN Kobe Campus, but also to accommodate a variety of animal research needs, including animal material and resource support, technical training, and education for proper conduct of animal research. In recent years, we have established colonies of a Soricomorpha species, suncus (Suncus murinus), a metatherian species, gray short-tail opossum (Monodelphis domestica), and a reptilian species, gecko (Paroedura picta) for prospective distribution services of these new resources to the research community. In addition, in close cooperation with Genetic Engineering Team, we are facilitating joint projects to generate and distribute novel genetically engineered mice with domestic and international biomedical research community, as well as conducting original research projects in live imaging and analyses of early mouse development.
Research Theme
- Advancement of technologies in reproductive biology
- In vitro development of early mouse embryos
Selected Publications
Nishiyama C, Saito Y, Sakaguchi A, et al.
Prolonged Myocardial Regenerative Capacity in Neonatal Opossum.
Circulation
146(2), 125-139 Tue Jul 12 00:00:00 JST 2022
doi: 10.1161/CIRCULATIONAHA.121.055269
Inada K, Hagihara M, Tsujimoto K, et al.
Plasticity of neural connections underlying oxytocin-mediated parental behaviors of male mice.
Neuron
110(12), 2009-2023 Wed Jun 15 00:00:00 JST 2022
doi: 10.1016/j.neuron.2022.03.033
Deviatiiarov R, Ishikawa K, Gazizova G, et al.
Integrative transcription start site analysis and physiological phenotyping reveal torpor-specific expression program in mouse skeletal muscle.
Communications Biology
4(1), 1290 Mon Nov 15 00:00:00 JST 2021
doi: 10.1038/s42003-021-02819-2
Kiyonari H, Kaneko M, Abe T, et al.
Targeted gene disruption in a marsupial, Monodelphis domestica, by CRISPR/Cas9 genome editing.
Current Biology
31(17), 3956-3963 Mon Sep 13 00:00:00 JST 2021
doi: 10.1016/j.cub.2021.06.056
Hirata T, Tohsato Y, Itoga H, et al.
NeuroGT: A brain atlas of neurogenic tagging CreER drivers for birthdate-based classification and manipulation of mouse neurons.
Cell Reports Methods
1, 100012 Mon Jul 26 00:00:00 JST 2021
doi: 10.1016/j.crmeth.2021.100012
Kiyokawa H, Yamaoka A, Matsuoka C, et al.
Airway basal stem cells reutilize the embryonic proliferation regulator, Tgfβ-Id2 axis, for tissue regeneration.
Developmental Cell
56(13), 1917-1929 Mon Jul 12 00:00:00 JST 2021
doi: 10.1016/j.devcel.2021.05.016
Minegishi K, Rothé B, Komatsu KR, et al.
Fluid flow-induced left-right asymmetric decay of Dand5 mRNA in the mouse embryo requires a Bicc1-Ccr4 RNA degradation complex.
Nature Communications
12(1), 4071 Thu Jul 01 00:00:00 JST 2021
doi: 10.1038/s41467-021-24295-2
Morita R, Sanzen N, Sasaki H, et al.
Tracing the origin of hair follicle stem cells.
Nature
594(7864), 547-552 Tue Jun 01 00:00:00 JST 2021
doi: 10.1038/s41586-021-03638-5
Abe T, Inoue KI, Furuta Y, Kiyonari H.
Pronuclear Microinjection during S-Phase Increases the Efficiency of CRISPR-Cas9-Assisted Knockin of Large DNA Donors in Mouse Zygotes.
Cell Reports
31(7), 107653 Tue May 19 00:00:00 JST 2020
doi: 10.1016/j.celrep.2020.107653
Kajikawa E, Horo U, Ide T, et al.
Nodal paralogues underlie distinct mechanisms for visceral left-right asymmetry in reptiles and mammals.
Nature ecology & evolution
4(2), 261-269 Sat Feb 01 00:00:00 JST 2020
doi: 10.1038/s41559-019-1072-2
Kiyonari H, Kaneko M, Abe T, et al.
Dynamic organelle localization and cytoskeletal reorganization during preimplantation mouse embryo development revealed by live imaging of genetically encoded fluorescent fusion proteins.
Genesis.
57(2), e23277 Sun Dec 01 00:00:00 JST 2019
doi: 10.1002/dvg.23277
Kime C, Kiyonari H, Ohtsuka S, et al.
Induced 2C Expression and Implantation-Competent Blastocyst-like Cysts from Primed Pluripotent Stem Cells.
Stem cell reports
13(3), 485-498 Tue Sep 10 00:00:00 JST 2019
doi: 10.1016/j.stemcr.2019.07.011
Nakao H, Harada T, Nakao K, et al.
A possible aid in targeted insertion of large DNA elements by CRISPR/Cas in mouse zygotes.
Genesis.
54(2), 65-77 Thu Dec 01 00:00:00 JST 2016
doi: 10.1002/dvg.22914
Yoshida M, Kajikawa E, Kurokawa D, et al.
Conserved and divergent expression patterns of markers of axial development in eutherian mammals.
Developmental Dynamics
245(1), 67-86 Tue Nov 01 00:00:00 JST 2016
doi: 10.1002/dvdy.24352
Katayama A, Nakatsuka A, Eguchi J, et al.
Beneficial impact of Gpnmb and its significance as a biomarker in nonalcoholic steatohepatitis.
Scientific Reports
5, 16920 Tue Dec 01 00:00:00 JST 2015
doi: 10.1038/srep16920
Hara Y, Tatsumi K, Yoshida M, et al.
Optimizing and benchmarking de novo transcriptome sequencing: from library preparation to assembly evaluation.
BMC Genomics
16(1), 977 Sun Nov 01 00:00:00 JST 2015
doi: 10.1186/s12864-015-2007-1
Popov LM, Marceau CD, Starkl PM, et al.
The adherens junctions control susceptibility to Staphylococcus aureus α-toxin.
Proceedings of the National Academy of Sciences of the United States of America
112(46), 14337-14342 Thu Oct 01 00:00:00 JST 2015
doi: 10.1073/pnas.1510265112
Nakatsu Y, Iwashita M, Sakoda H, et al.
Prolyl isomerase Pin1 negatively regulates AMP-activated protein kinase (AMPK) by associating with the CBS domain in the γ subunit.
The Journal of Biological Chemistry
290(40), 24255-24266 Tue Sep 01 00:00:00 JST 2015
doi: 10.1074/jbc.M115.658559
Hirate Y, Hirahara S, Inoue K.
Potential biomarkers of fatigue identified by plasma metabolome analysis in rats.
Development, Growth & Differentiation
57(8), 544-556 Sat Aug 01 00:00:00 JST 2015
doi: 10.1111/dgd.12235
Imai Y, Kobayashi Y, Inoshita T, et al.
The Parkinson's Disease-Associated Protein Kinase LRRK2 Modulates Notch Signaling through the Endosomal Pathway.
PLOS Genetics
16(5), 517-532 Wed Jul 01 00:00:00 JST 2015
doi: 10.1371/journal.pgen.1005503
Cui YL, Toyoda H, Sako T, et al.
A voxel-based analysis of brain activity in high-order trigeminal pathway in the rat induced by cortical spreading depression.
Neuroimage
108, 17-22 Mon Jun 01 00:00:00 JST 2015
doi: 10.1016/j.neuroimage.2014.12.047
Lee MS, Hwang KS, Oh HW, et al.
IFT46 plays an essential role in cilia development.
Developmental Biology
400(2), 248-257 Fri May 01 00:00:00 JST 2015
doi: 10.1016/j.ydbio.2015.02.009
Members
Hiroshi Kiyonari
Team Leader
Takaya Abe
Technical Scientist
Kenichi Inoue
Expert Technician
Mayo Shigeta
Expert Technician
Mari Kaneko
Technical Staff
Miho Sato
Technical Staff
Aki Shiraishi
Technical Staff
Tomoko Tokunaga
Technical Staff
Tomoko Tokuhara
Technical Staff
Arisa Tobe
Technical Staff
Kohei Nakano
Technical Staff
Miwako Nomura
Technical Staff
Kana Bando
Technical Staff
Michiko Higashikawa
Technical Staff
Daisuke Yamamoto
Technical Staff
Riko Yoshimi
Technical Staff
Megumi Watase
Technical Staff
Masayo Okugawa
Assistant