Team Leader
Atsuko Iwane Ph.D.

Laboratory for Cell Field Structure

[Closed Mar. 2023]

E-mailatsuko.iwane[at]riken.jp

Please replace [at] with @.

Overview
Selected Publications

Electron microscopy (EM) offers the best in resolution of extremely small biological specimens, providing images of the minutest of organelles and molecules that are responsible for phenomena fundamental to life including cell division, differentiation and proliferation. The resulting fine structural information has been extraordinary in helping our understanding of how morphology and function relate. 　We seek to image several target molecules and organelles, including the mitochondria and chloroplasts, and their surrounding environments inside a cell by using two different electron microscopy technologies. The first is Cryo-EM tomography, which produces a 3D image by scanning the field of view at multiple angles over an angular range from -70 degrees to 70 degrees relative to the perpendicular of the specimen plane used in transmission electron microscopy (TEM). The second is focused ion beam scanning electron microscopy (FIB-SEM), which uses SEM to scan a number of sections that are created by FIB. In this aim, we will combine our electron microscopy system with fluorescence microscopy to conduct dual imaging of the specimen in the same field of view. This will provide information on the dynamics of the specimen, which will complement the structure information and give us better understanding of the relationship between cell fate and cell morphology.

Research Theme

Development of stainless cell imaging by Cryo-TEM
Whole cell structure reconstruction by three-dimensional FIB-SEM

Selected Publications

Mizuno K, Shiozawa K, Katoh TA, et al.
Role of Ca²⁺ transients at the node of the mouse embryo in breaking of left-right symmetry.
Science Advances 6, eaba1195 Tue Dec 01 00:00:00 JST 2020 doi: 10.1126/sciadv.aba1195

Miyamoto T, Hosoba K, Itabashi T, et al.
Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.
The EMBO journal 39(12), e103499 Wed Jun 17 00:00:00 JST 2020 doi: 10.15252/embj.2019103499

Fujii T, Iwane AH, Yanagida T, Namba K.
Direct visualization of secondary structures of F-actin by electron cryomicroscopy.
Nature 467(7316), 724-728 Wed Dec 01 00:00:00 JST 2010 doi: 10.1038/nature09372

Nishikawa S, Arimoto I, Ikezaki K, et al.
Switch between Large Hand-Over-Hand and Small Inchworm-like Steps in Myosin VI.
Cell 142(6), 879-888 Mon Nov 01 00:00:00 JST 2010 doi: 10.1016/j.cell.2010.08.033

Watanabe TM, Yanagida T, Iwane AH.
Single molecular observation of self-regulated kinesin motility.
Biochemistry 49, 4654-4661, Fri Oct 01 00:00:00 JST 2010 doi: 10.1021/bi9021582

Iwane AH, Morimatsu M, Yanagida T.
Recombinant alpha-actin for specific fluorescent labeling.
Proceedings of the Japan Academy Series B-Physical and Biological Sciences 85(10), 491-499 Tue Dec 01 00:00:00 JST 2009 doi: 10.2183/pjab.85.491

Iwaki M, Iwane AH, Shimokawa T, et al.
Brownian search-and-catch mechanism for myosin-VI steps.
Nature Chemical Biology 5(6), 403-405 Sun Nov 01 00:00:00 JST 2009 doi: 10.1038/nchembio.171

Iwane AH. Tanaka H, Morimoto S, et al.
The neck domain of myosin II primarily regulates the actomyosin kinetics, not the 10.1016/j.jmb.2005.08.013stepsize.
Journal of Molecular Biology 353, 213-221, Thu Dec 01 00:00:00 JST 2005 doi: 10.1016/j.jmb.2005.08.013

Tanaka H, Homma K, Iwane AH, et al.
The motor domain determines the large step of myosin-V.
Nature 415, 192-195, Sun Dec 01 00:00:00 JST 2002 doi: 10.1038/415192a

Kitamura K, Tokunaga M, Iwane AH, Yanagida T.
A single myosin head moves along an actin filament with regular steps of 5.3 nanometres.
Nature 397, 129-134, Wed Dec 01 00:00:00 JST 1999 doi: 10.1038/16403

Iwane AH, Funatsu T, Harada Y, et al.
Single molecular assay of the individual ATP turnovers by a myosin-GFP fusion protein expressed in vitro.
FEBS Letters 407, 235-238, Mon Dec 01 00:00:00 JST 1997 doi: 10.1016/S0014-5793(97)00359-1

Iwane AH, Kitamura K, Tokunaga M, Yanagida T.
Myosin subfragment-1 is fully equipped with factors essential for motor function.
Biochemical and Biophysical Research Communications 230, 76-80, Sat Nov 01 00:00:00 JST 1997 doi: 10.1006/bbrc.1996.5861

Team LeaderAtsuko Iwane Ph.D.

Research Theme

Selected Publications

Team Leader
Atsuko Iwane Ph.D.