Laboratory for Organismal Patterning
Hiroshi HamadaM.D., Ph.D.
- Location：Kobe / Developmental Biology Buildings
- E-mail：hiroshi.hamada[at]riken.jpPlease replace [at] with @.
My lab studies how left-right asymmetries develop in the mouse embryo. In particular, we focus on two types of cilia that are required for left-right symmetry breaking: rotating cilia that generate leftward fluid flow, and immotile cilia that sense the fluid flow. We also study the role of maternal epigenetic regulators in development. We address these questions by integrating live imaging, structural biology, fluid dynamics and mathematical modeling.
How left-right asymmetry is established in vertebrates
Role of motile and immotile cilia in left-right symmetry breaking
Maternal and paternal epigenome in development
- How motion pattern of node cilia is determined
- How immotile cilia sense fluid flow
- Role of maternal and paternal epigenetic factors in development
Main Publications List
Nabeshima R, Nishimura O, Maeda T, et al.
A Switch-like Activation Relay of EGFR-ERK Signaling Loss of Fam60a, a Sin3a subunit, results in embryonic lethality and is associated with aberrant methylation at a subset of gene promoters.
eLife 7, e36435(2018) doi: 10.7554/eLife.36435
Takaoka K, Nishimura H and Hamada H.
Both Nodal signaling and stochasticity select for prospective distal visceral endoderm in mouse embryos.
Nature Communications 8. 1492 (2017) doi :10.1038/s41467-017-01625-x
Minegishi K, Hashimoto M, Ajima R, et al.
A Wnt5 activity asymmetry and intercellular signaling polarize node cells for breaking left-right symmetry in the mouse embryo.
Developmental Cell 40. 439–452 (2017) doi:10.1016/j.devcel.2017.02.010
Shinohara K, Chen D, Nishida T, et al.
Absence of radial spokes in mouse node cilia is required for rotational movement but confers ultrastructural instability as a trade-off.
Developmental Cell 35. 236–246 (2015) doi:10.1016/j.devcel.2015.10.001
Nakamura T, Saito D, Kawasumi A, et al.
Fluid flow and interlinked feedback loops establish left-right asymmetric decay of Cerl2 mRNA in the mouse embryo.
Nature Communications 3. 1322 (2012) doi:10.1038/ncomms2319
Yoshiba S, Shiratori H, Kuo I Y, et al.
Cilia at the node of mouse embryos sense fluid flow for left-right determination via Pkd2. Science 338. 226–231 (2012) doi:10.1126/science.1222538