Team Leader
Hiroshi Hamada M.D., Ph.D.

Laboratory for Organismal Patterning

[Closed Mar. 2023]

E-mailhiroshi.hamada[at]riken.jp

Please replace [at] with @.

Overview
Selected Publications
News

My lab studies how left-right asymmetries develop in the mouse embryo. In particular, we focus on two types of cilia that are required for left-right symmetry breaking: rotating cilia that generate leftward fluid flow, and immotile cilia that sense the fluid flow. We also study the role of maternal epigenetic regulators in development. We address these questions by integrating live imaging, structural biology, fluid dynamics and mathematical modeling.

How left-right asymmetry is established in vertebrates

Role of motile and immotile cilia in left-right symmetry breaking

Maternal and paternal epigenome in development

Research Theme

How motion pattern of node cilia is determined
How immotile cilia sense fluid flow
Role of maternal and paternal epigenetic factors in development

Selected Publications

Mizuno K, Shiozawa K, Katoh TA, et al.
Role of Ca²⁺ transients at the node of the mouse embryo in breaking of left-right symmetry.
Science Advances 6, eaba1195 Tue Dec 01 00:00:00 JST 2020 doi: 10.1126/sciadv.aba1195

Kajikawa E, Horo U, Ide T, et al.
Nodal paralogues underlie distinct mechanisms for visceral left-right asymmetry in reptiles and mammals.
Nature Ecology & Evolution 4, 261-269 Sun Nov 01 00:00:00 JST 2020 doi: 10.1038/s41559-019-1072-2

Nabeshima R, Nishimura O, Maeda T, et al.
Loss of Fam60a, a Sin3a subunit, results in embryonic lethality and is associated with aberrant methylation at a subset of gene promoters.
eLife 7, e36435 Thu Aug 02 00:00:00 JST 2018 doi: 10.7554/eLife.36435

Takaoka K, Nishimura H, Hamada H.
Both Nodal signaling and stochasticity select for prospective distal visceral endoderm in mouse embryos.
Nature Communications 8, 1492 Fri Dec 01 00:00:00 JST 2017 doi: 10.1038/s41467-017-01625-x

Minegishi K, Hashimoto M, Ajima R, et al.
A Wnt5 activity asymmetry and intercellular signaling polarize node cells for breaking left-right symmetry in the mouse embryo.
Developmental Cell 40, 439-452 Wed Nov 01 00:00:00 JST 2017 doi: 10.1016/j.devcel.2017.02.010

Shinohara K, Chen D, Nishida T, et al.
Absence of radial spokes in mouse node cilia is required for rotational movement but confers ultrastructural instability as a trade-off.
Developmental Cell 35, 236-246 Tue Dec 01 00:00:00 JST 2015 doi: 10.1016/j.devcel.2015.10.001

Nakamura T, Saito D, Kawasumi A, et al.
Fluid flow and interlinked feedback loops establish left-right asymmetric decay of Cerl2 mRNA in the mouse embryo.
Nature Communications 3, 1322 Sat Dec 01 00:00:00 JST 2012 doi: 10.1038/ncomms2319

Yoshiba S, Shiratori H, Kuo IY, et al.
Cilia at the node of mouse embryos sense fluid flow for left-right determination via Pkd2.
Science (New York, N.Y.) 338(6104), 226-31 Fri Oct 12 00:00:00 JST 2012 doi: 10.1126/science.1222538